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Lysis or Lysogeny ? Gene Regulation by CI (and CI like) Repressor Proteins

Lysis or Lysogeny ? Gene Regulation by CI (and CI like) Repressor Proteins. By Firras Garada. Introduction. A lysogenic state is when a viral genome is inserted and integrated into the host genome and is replicated when the host DNA divides

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Lysis or Lysogeny ? Gene Regulation by CI (and CI like) Repressor Proteins

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  1. Lysis or Lysogeny? Gene Regulation by CI (and CI like) Repressor Proteins By Firras Garada

  2. Introduction • A lysogenic state is when a viral genome is inserted and integrated into the host genome and is replicated when the host DNA divides • A lytic state is when phage replication leads to newly replicated phages, cell lysisand release of different phage progeny • It has been shown that a specific type of lactococcal phage (TP901-1) has a CI repressor protein (TP901-1p03) that controls whether the phage enters a lysogenic or lytic pathway

  3. Research Question and Hypothesis • Do other phages besides Lactoccous TP901-1 have a gene similar to the CI repressor gene that regulates between the lysogenic or lytic pathway? • I suspect yes that other phages do have a gene that is like the CI repressor gene – and these genes are what I seek to find

  4. Methods • Using the biobike interface I first found the CI repressor gene in the TP901-1 phage. • Then I used the proteins similar to function and genes similar to function to find proteins in all phages (stored in biobike) that are similar to the CI repressor gene • Also used a list of genes that someone had created (subset) in my analysis

  5. Method continued • Analysis came from use of Motif

  6. Results • Motif Summary:

  7. Conclusion • The CI repressor protein in TP901-1 that was described to regulate lysogeny/lytic pathway is TP901-1p03. • As the motif results show there are 6 other proteins that have all 3 motifs that TP901-1p03 does. • This shows that these proteins are conserved and have sequences very similar to TP901-1p03. • In conclusion I would say it’s possible that these 6 proteins play a regulatory role like TP901-1p03 but further analysis is needed to know for sure.

  8. Work Cited • Characterization of the CI Repressor Protein Encoded by the Temperate Lactococcal Phage TP901-1 Margit Pedersen, MałgorzataLigowska, Karin Hammer J Bacteriol. 2010 April; 192(8): 2102–2110. Published online 2010 January 29. doi: 10.1128/JB.01387-09 • http://biobike.csbc.vcu.edu/

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