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pcr gel

pcr gel. Tucson High School Biotechnology Course Spring 2010. What is gel electrophoresis?. -. -. -. -. -. -. -. wells. DNA (-). +. +. +. +. +. +. +. pcr products. Loading Buffer. STAIN. DNA Sequencing. DNA Sequencing: Basic Principle. Sequence of base pairs??. A. G.

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pcr gel

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  1. pcr gel Tucson High School Biotechnology Course Spring 2010

  2. What is gel electrophoresis? - - - - - - - wells DNA (-) + + + + + +

  3. + pcr products Loading Buffer STAIN

  4. DNA Sequencing

  5. DNA Sequencing: Basic Principle Sequence of base pairs?? A G G T C A A G C T A T C polymerase

  6. The quest for the $1K human genome 2nd: 10,000 instrument days (30 instruments for 1 year) @ $10 million 3rd: 454 pyrosequencing, two months @ $2 million J. Craig Venter James Watson • Nature, Nov 2008 -- “effectively” 4 human genomes (@$100K each) • first African genome (Illumina, UK) • first Asian genome (Beijing Genomics, China) • first cancer genome (Washington University, USA) first human genome = ten years @ $1 billion The $1,000 human genome, coming soon?

  7. 454 Pyrosequencing - the generations

  8. The “pyro” in pyrosequencing http://www.pyrosequencing.com/

  9. Pyrosequencing - Library construction Images courtesy of Roche (Technical presentation)

  10. Pyrosequencing - emPCR

  11. Pyrosequencing - sequencing

  12. Pyrosequencing - image processing

  13. Sequencing costs 3.1 2.0 1.00 1.8 0.90 1.6 0.80 1.4 0.70 1.2 0.60 1.0 0.50 .8 0.40 .6 0.30 .4 0.20 .2 0.10 0 0 Sequence production (Billions of bases/month) Cost: Cents per base 2003 2005 1995 2001 1997 1999 1993 1989 1991 Courtesy of Phil Hugenholtz (JGI)

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