Analysis of Mutated Recombinant Proteins via SDS-PAGE: Purification Procedure

Jan 12, 2025

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This figure presents SDS-PAGE results of mutated recombinant proteins in the purification process. Fractions eluted from a heparin column were loaded and revealed by Coomassie staining using molecular weight markers.

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Analysis of Mutated Recombinant Proteins via SDS-PAGE: Purification Procedure

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Fig S1 IN 143R/97A IN 143C/97A IN 97A IN 143C IN 143R IN WT (kDa) MW 100 70 55 35 IN 25 15 10 Figure S1.SDS-PAGE of mutated recombinant proteins used in this study. Proteins obtained in the purification procedure were analyzed on SDS-PAGE and revealed by coomassie staining. 5 µL fractions eluted from heparin column were loaded on the gel. MW, molecular weight markers (PageRuler Prestained protein ladder plus, Fermentas).