Enterobacteriaceae

1. Enterobacteriaceae Slackers Facts by Mike Ori

2. Disclaimer The information represents my understanding only so errors and omissions are probably rampant. It has not been vetted or reviewed by faculty. The source is our class notes. The document can mostly be used forward and backward. I tried to mark questionable stuff with (?). If you want it to look pretty, steal some crayons and go to town. Finally… If you’re a gunner, buck up and do your own work.

3. Enterobacteriacea characteristics

4. Rapidly growing large gram negative rods of various sizes that can grow on plain agar both aerobically or anaerobically.

5. Enterobacteriaceae antigens

6. O: outer membrane lipopolysaccharide H: flagellar K: cell surface polysaccharides

7. Enterobacteriacaea common pathogens

8. Escherichia Shigella Salmonella Klebsiella Yersinia

9. Enterobacteriaceae uncommon pathogens

10. Enterobacter Serratia Proteus Morganella Providencia

11. Most common Enterobacteriaceae species

12. Escherichia coli

13. Enterobacteriaceae dedicated pathogens

14. Shigella Salmonella

15. E Coli type 1 pili characteristics

16. Pili that mediates general attachment to a wide variety of epithelial cells via adhesins that interact with host D-mannose residues

17. E. Coli P pili characteristics

18. Pili that interact with digalactose (gal-gal) residues on uroepithelium thus allowing efficient colonization of the urinary tract.

19. E. coli colonizing factor antigen (CFA) or bundle forming pilin (BFP) characteristics

20. Pili that mediate attachment to enterocytes

21. E. Coli alpha hemolysin toxin

22. Pore forming exotoxin

23. Shiga toxin

24. Interferes with protein synthesis: An A-B toxin secreted by E. coli whose active unit interferes with protein synthesis by blocking the ribosomal amino acyltRNA binding site. Causes hemolysis by unknown mechanisms. Interferes with kidney function: Circulating toxin binds renal tissues causing glomerular swelling and the deposition of fibrin and platelets into the microvasculature.

25. Labile toxin

26. Cholera-like A-B toxin generated by some E. coli strains that increases cAMP production in host cells by ADP-ribosylation of Gas

27. Stable toxin

28. Cholera-like (A-B?) toxin generated by some E. coli strains that increases cGMP production by host cells

29. E. Coli fancy smancy names

30. UPEC – uropathogenic EC ETEC – enterotoxigenic EC EPEC – enteropathogenic EC EHEC – enterohemorrhagic EC EIEC – enteroinvasive EC

31. E. Coli UTI causation %

32. 90% of UTIs

33. UTI common patient

34. Sexually active women

35. E. Coli UTI pathogenicity factors

36. Type 1 pili mediate binding in periurethral area. P pili mediate attachment to urogenital cells.

37. ETEC characteristics

38. Watery diarrhea caused by labile/stable toxins with bacterial attachment facilitated by CFA (colony factor antigen) pili. All of the above are plasmid borne.

39. ETEC epidemiology

40. Significant cause of mortality in children 2 and younger in less developed countries. Common cause of traveler’s diarrhea. Human reservoir. Point to ponder: What kind of diarrhea would the time traveler’s wife’s husband have?

41. ETEC symptoms

42. Watery diarrhea

43. EPEC characteristics

44. Bundle forming pili (BFP) bearing intimin protein mediate attachment of the cells to host enterocytes. Subsequent injection of E. coli secretion proteins (Esp) causes the formation of a pedestal by modifying the cytoskeleton.

45. Attachment and effacing lesion

46. Lesion caused by EPEC and EHEC E. coli strains by injection of E. coli secretory proteins (Esp) that results in the effacement of enterocyte microvilli and the attachment of E. coli at the top of pedestals formed from commandeered cytoskeletal components.

47. Whats the coolest thing about intimin

48. E. coli injects the receptor for intimin along with E. coli secretion proteins (Esp)

49. EPEC epidemiology

50. Common in less developed countries where it accounts for 20% of the diarrhea in bottle fed infants below 1 year of age.