Biology 22

1. Biology 22 Drosophila Identification of various phenotypes.

2. Outline • Brief description of Drosophila phenotypes and life cycle • 1-Experiment 1: collection of data related to gender and body color • 2-Experiment : 3 point Test Cross • How to study keep your flies still during the identification.

3. Reference for Pictures: • Carolina Drosophila melanogster Manual (45-2620) • Published: Carolina Biological Supply Company • Based on research of Dr. Thomas Hunt Morgan

4. 1866-1945

5. The life Cycle

6. Life Cycle:Drosophila melanogaster • Eggs: contain filaments • 2 molting phases “instars” • Life cycle is 10 days

7. Fruity Fly Egg and Larva

8. Fruit Fly Pupa and Adult

9. 1- EXPERIMENT: Identification of: • Gender • Body Color

10. Wild Type Versus Mutants • Body Color • Wild type: Beige • Mutant: Yellow body

11. Parental phenotypes Experiment: body color • A= female: wt (beige) • B=female: wt (beige) • C=male: wt (beige) • D=female: mutant (yellow) • E=male: mutant (yellow)

12. Fruit Fly Body Color

13. Sex combs: Male Drosophila

14. Female and Male Fruit Flies

15. Abdominal segements • 7 segments to female abdominal segments • 5 segments to male abdominal segments • Male body size is smaller than female • But the “last” abdominal segment is larger in male

17. Experiment 2 3 point test cross Eye color Body color Wing length 2- Experiment: 3 point test cross:

18. 1. Body Color 2. Wing length 3. Eye Color ♦Wt vs. Mutant Beige (bl+) vs. Black (bl) Long(vg+) vs. Vestigal(vg) Brick red (br+) vs. brown (br) 3 point test cross for:

19. Crosses: Female Males • Males and female progeny from this F2 cross are your 3 point test cross results! bl+ vg+ Bw+ bl vg bw X bl vg bw bl vg bw

20. There are all sorts of eyes!

21. Eyes

22. You will have only red eyes (wt)

23. Fruit Fly Wings

24. Nap TimeTriethylamine

25. How to anesthetize a fly

26. How to use the Napper

27. View the Fruit Fly

28. You will record class data • After counting the crosses record the data on the class spreadsheet