Biology 22

1. Biology 22 Drosophila Identification of various phenotypes.

2. Reference for Pictures: • Carolina Drosophila melanogster Manual (45-2620) • Published: Carolina Biological Supply Company

3. The life Cycle

4. Life Cycle:Drosophila melanogaster • Eggs: contain filaments • 2 molting phases “instars” • Life cycle is 10 days

5. Fruity Fly Egg and Larva

6. Fruit Fly Pupa and Adult

7. Identification of: • Gender • Body Color

8. Wild Type Versus Mutants • Body Color • Wild type: Beige • Mutant: Yellow body

9. Parental phenotypesExperiment: body color • A= female: wt (beige) • B=female: wt (beige) • C=male: wt (beige) • D=female: mutant (yellow) • E=male: mutant (yellow)

10. Fruit Fly Body Color

11. Sex combs: Male Drosophila

12. Abdominal segements • 7 segments to female abdominal segments • 5 segments to male abdominal segments • Male body size is smaller than female • But the “last” abdominal segment is larger in male

13. Nap TimeTriethylamine

14. How to anesthetize a fly

15. How to use the Napper

16. View the Fruit Fly

17. You will record class data • After counting the crosses record the data on the class spreadsheet

19. Experiment 2 3 point test cross Eye color Body color Wing length 3 point test cross: Experiment 2

20. 1. Body Color 2. Wing length 3. Eye Color ♦Wt vs. Mutant Beige (bl+) vs. Black (bl) Long(vg+) vs. Vestigal(vg) Brick red (br+) vs. brown (br) 3 point test cross for:

21. Crosses: Female Males • Males and female progeny from this F2 cross are your 3 point test cross results! bl+ vg+ Bw+ bl vg bw X bl vg bw bl vg bw

22. There are all sorts of eyes!

23. Eyes

24. You will have only red eyes (wt)

25. Fruit Fly Wings

26. 1866-1945

29. Parental phenotypes • A= female: wt beige body • B=female: wt beige body • C=male: wt beige body • D=female: yellow body • E=male: yellow body

30. Female and Male Fruit Flies