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Understanding Blood Constituents: Plasma, Cells, and Functions

Explore the components of blood, including plasma, erythrocytes, leukocytes, and platelets. Learn about their characteristics, functions, and staining methods used for identification.

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Understanding Blood Constituents: Plasma, Cells, and Functions

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  1. blood

  2. Constituents of blood water（90%） others plasma （55%） blood cell （45%） blood Ery Leu Blood platelet

  3. (erythrocyte,red blood cell,RBC) staining method：Wright or Giemsa.

  4. SEM

  5. Erythrothyte • size：Diameter7.5-8.5 um. • shape：biconcave circular disc • morphology：no nuclei& other organelles, varieties. • ABO Antigen: blood group. • contents：haemoglobin(Hb)。 • amount： 35-55lakh/mm3。

  6. Reticulocyte • premature RBC:left some ribosome in the cytoplasm. • Amount:0.5%-1.5%. • Can synthesize Hb.

  7. leukocytes(white blood cell, WBC) neutrophils(60-70%) eosinophils(3%) basophils(1%) granulocytes Agranular leukocytes WBC lymphocytes(20-30%) monocytes(5%) Depending on what?

  8. amount：60-70 %. • Dia : 10-12um. • nuclei：variable. roddish & several lobes（2-5）. • Granules: fine, stain lightly. • functions：chemotaxis & phagocytosis. （LM） Neutrophilic granulocyte

  9. （EM） Neutrophilic granulocyte Azurophilic granules Nuclear lobes specific granules

  10. amount：0.5-3%. • Dia:10-15um. • nuclei：two-lobes. • granules：large, stain brightly withacid dyes. • Functions:chemotaxis & amoeban movement. • *inhibit allergic reaction. • *kill parasite. (LM) Eosinophilic granulocyte

  11. (EM) Eosinophilic granulocyte crystalloid

  12. amount：0-1%. • Dia:10-12 um. • nuclei：irregular, shaped “S”. • granules：metachromatical, purple-blue,ir- • regular in size & distribution. ( LM ) Basophilic granulocyte

  13. ( EM ) Basophilic granulocyte

  14. Amount：20-30%. • Dia: virable. • nuclei：large，spherical,indentation on one side,stain densely. • cytoplasm：scanty, clear blue. • Classification: • TC BC KC NKC （LM） Lymphocyte

  15. （EM） Lymphocyte

  16. Amount：5%. • size：the biggest cells in blood.， • nuclei：kidney,horse-shoe. • cytoplasm：abundant,basic, grey blue. • Mononuclear phagocyte system. （LM） Monocyte

  17. （EM） Monocyte Azurophilic granules microvili

  18. Are small masses of cytoplasm shed from megakaryocyte. • Irregular disc, • biconvex. No nuclei,In groups . • A granular central part. • A clear outer zone. • Functions: blood clotting & coagulation. （LM） Blood platelet

  19. （EM） Blood platelet 特殊颗粒 Granular part Clear area

  20. Review!!

  21. LM ??? ???

  22. EM Neutrophil!!!

  23. LM • Large • Horseshoe • nucleus

  24. LM • monocyte！！！

  25. LM

  26. EM Eosinophil!

  27. LM ???

  28. EM Lymphocyte!!!

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