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Genetic Engineering

Genetic Engineering. Review Game. Best Arnold character. 1. 3. Terminator Predator Mr. Freeze Kindergarten Cop. 2. 4. DNA that is from two or more organisms is called…. Combinant DNA Remade DNA Recombinant DNA Multiple DNA. Fastest Responders (in seconds). Today’s Topics.

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Genetic Engineering

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  1. Genetic Engineering Review Game

  2. Best Arnold character 1. 3. • Terminator • Predator • Mr. Freeze • Kindergarten Cop 2. 4.

  3. DNA that is from two or more organisms is called… • Combinant DNA • Remade DNA • Recombinant DNA • Multiple DNA

  4. Fastest Responders (in seconds)

  5. Today’s Topics Genetic Engineering GMOs and Cloning Gel Electrophoresis Stem Cells Final Results

  6. Genetic Engineering

  7. Which of these is not an example of a vector used in genetic engineering? • Plasmid • Yeast • Virus • Bacteria

  8. What is used to cut DNA? • Restriction Enzymes • DNA Ligase • Scissors • Recombinant Enzymes

  9. If I wanted to produce human insulin using a transformed bacteria, what would I need to do first? • Recombine the DNA with a vector • Cut out the gene of interest • Isolate the gene of interest • Insert the gene into the bacteria

  10. I have now made my Recombinant DNA. What must take place before I can clone the gene? • Transform the bacteria • Select and screen • Gene cloning • Use DNA Ligase

  11. DNA fragments cut with EcoRI can be combined with DNA cut with • BamHI • HindIII • EcoRI • All of the above • 1 & 2 • 2 & 3

  12. Restriction fragments cut with a restriction enzyme have a single- stranded 4 base tail of AATT. What is this called? • Blunt end • Sticky end • Restriction tail • Ligase

  13. How does the gene get cloned once it has been taken in by the bacteria? • Using PCR • Do more transformations • Bacterial reproduction • Gel electrophoresis

  14. Once I have selected and screened the bacteria, I can now … • Extract the gene • Extract the protein • Give the bacteria to an ill person • All of these • 1 & 2 • 1 & 3

  15. Fastest Responders (in seconds)

  16. Team Scores

  17. GMOs and Cloning FASTEST FINGER

  18. Which is easier to clone? • Animals • Plants • Humans

  19. Which of these is not a reason for making crops GMOs? • Better Taste • Drought Resistance • Pest Resistant • All of the above • Only 2 and 3

  20. If I wanted to clone a sheep 1, using an egg from sheep 2, and having sheep 3 deliver the baby, where do I get the DNA from? • Sheep 1 • Sheep 2 • Sheep 3

  21. The method of cloning described in the last slide is called? • Nuclear replacement • Somatic cell nuclear transplantation • Artificial twinning • Egg cell fertilization

  22. Which type of cell can I get the DNA from? • Heart cell • Skin cell • Liver cell • Muscle cell • None of these • All of these

  23. Fastest Responders (in seconds)

  24. Team Scores

  25. Gel Electrophoresis FASTEST FINGER

  26. Gel Electrophoresis does what? • DNA Fingerprinting • Paternity testing • Separates molecules by size • Uses electricity

  27. Since everyone on the planet has different DNA, identical twins excluded, • No one has the same DNA fingerprint • Most people have a different DNA fingerprint • You are less likely to have the same DNA fingerprint as another

  28. In RFLP analysis, with what do we mix our DNA samples before running them through a gel • DNA ligase • plasmids • Restriction enzymes • DNA polymerase

  29. Who is the baby’s father? • Bob • Larry • Neither

  30. Which direction would DNA move while being separated in gel electrophoresis? • Towards the positive • Towards the negative • Both directions depending on the person

  31. Which DNA Fragment is shorter? • A • B

  32. Who matches the blood stain? • Bob • Sue • John • Lisa

  33. PCRs main function is to • Exponentially Amplify DNA • Make a few copies of DNA • Catch criminals • Extract DNA

  34. Fastest Responders (in seconds)

  35. Team Scores

  36. Stem Cells

  37. Which of these can differentiate into the widest variety of cells? • Totipotent • Multipotent • Pluripotent

  38. Embryonic stem cells are • Totipotent • Pluripotent • Multipotent

  39. Where do most adult stem cells used in medicine come from? • Kidneys • Skin • Blood • Bone marrow

  40. Adult stem cells are • Totipotent • Pluripotent • Multipotent

  41. Which type of stem cell is created from a differentiated somatic cell? • Adult stem cells • Embryonic stem cells • Induced pluripotent stem cells

  42. Fastest Responders (in seconds)

  43. Team Scores

  44. Team MVP

  45. Participant Scores

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