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Microscope Notes

Microscope Notes. Parts of a microscope. 1. Eye piece/ocular- the piece that is looked through, has a power of 10. 2. Body tube- skinny tube attached to the eye piece that allows light to shine from the light source. 3. Nose piece- rotates the objective lenses

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Microscope Notes

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  1. Microscope Notes

  2. Parts of a microscope • 1. Eye piece/ocular- the piece that is looked through, has a power of 10. • 2. Body tube- skinny tube attached to the eye piece that allows light to shine from the light source. • 3. Nose piece- rotates the objective lenses • 4. Objective lens (high, medium and low) used to magnify the specimen on the stage. • High power (longest lens = 40X) • Medium power (middle lens = 10X) • Low power (shortest lens = 4X)

  3. 5. Stage- the slide is placed here. • 6. Stage clips- holds the slide in place • 7. Aperture- hole in the stage that allows light to pass through the specimen. • 8. Diaphragm- thin disk below the stage that adjusts the amount of light needed. • 9. Legs/base- holds the entire microscope up.

  4. 10. Light source- provides light to see the object. • 11. Course adjustment- large knob that brings the stage closer to the objectives. Focuses the specimen. • 12. Fine adjustment- smaller knob that fine tunes the image. • 13. Arm- attaches the upper portion of the microscope to the base.

  5. 14. Slide- the rectangular piece that holds the specimen. • 15. Cover slip- the plastic square piece that covers the specimen.

  6. Microscope Rules • 1. When carrying the microscope you must carry it with the ARM and the BASE. • 2. The microscope must always be stored with the lowest powered objective in place. • 3. When bringing an object into focus you must use the lowest powered objective and the course adjustment. • 4. ONLY use the FINE adjustment on the highest powered objective. • 5. Lenses should only be cleaned with lens paper. • 6. The whole microscope must be kept away from the edge of the lab table.

  7. Creating a wet mount slide • 1. Obtain a slide • 2. Place the specimen on the center of the slide • 3. Place ONE or TWO drops of water ON the specimen • 4. Take the corner of the cover slip and place it near the drop of water and CAREFULLY lower the cover slip on to the water and specimen. • **IF you see perfectly round, white circles when looking through the eye piece THEN you are observing air bubbles  • ** Slides and cover slips may be cleaned with water and a paper towel.

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