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Use of dialysis to separate small and large molecules

Use of dialysis to separate small and large molecules. Gel filtration chromatography. Molecular mass determination by gel filtration chromatography. Some commonly used gel filtration materials. Ion-exchange chromatography with stepwise elution.

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Use of dialysis to separate small and large molecules

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  1. Use of dialysis to separate small and large molecules

  2. Gel filtration chromatography

  3. Molecular mass determination by gel filtration chromatography

  4. Some commonly used gel filtration materials

  5. Ion-exchange chromatography with stepwise elution

  6. Device for generating a linear concentration gradient

  7. Molecular formulas of cellulose-based ion exchangers

  8. Some biochemically useful ion exchangers

  9. Affinity chromatography

  10. Covalent linking of ligand to agarose

  11. Derivatization of epoxy-activated agarose

  12. Purification of staphylococcal nuclease by affinity chromatography on bisphosphothymidine-linked agarose

  13. Experimental arrangement for paper chromatography

  14. Two-dimensional paper chromatography

  15. Paper electrophoresis

  16. Apparatus for slab gel electrophoresis

  17. Diagram of a disc electrophoresis apparatus

  18. Polymerization of acrylamide and N,N¢-methylenebisacrylamide to form a cross-linked polyacryl-amide gel

  19. SDS-PAGE

  20. Logarithmic relationship between the molecular mass of a protein and its relative electrophoretic mobility in SDS-PAGE

  21. General formula of the ampholytes used in isoelectric focusing

  22. Isoelectric points of several common proteins

  23. Purification of rat liver glucokinase

  24. END

  25. An enzyme-linked immunosorbent assay (ELISA)

  26. Solubilities of several proteins in ammonium sulfate solutions

  27. Solubility of caboxy-hemoglobin at is isoelectric point as a function of ionic strength and ion type

  28. Solubility of b-lactoglobin as a function of pH at several NaCl concentrations

  29. Protein crystals

  30. Agarose gel electrophoretogram of a double helical DNA

  31. Pulsed-field gel electrophoresis (PFGE) of a set of different sized bacteriophage DNAs

  32. Detection of proteins by immunoblotting

  33. Two-dimensional (2D) gel electrophoresis

  34. Sedimentation coefficients in Svedbergs (S) for some biological materials

  35. A selection of preparative ultracentrifuge rotors

  36. Zonal ultracentrifugation

  37. Equilibrium density distribution of a CsCl solution in an ultracentrifuge spinning at 39,460 rpm

  38. Isopycnic ultracentrifugation

  39. Physical constants of some proteins

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