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Insoluble

S.2. A. B. MDA-MB-231. PaTu2. MDA-MB-231. e.v. PRKD2-wt. e.v. PRKD2-wt. e.v. PRKD2-wt. p =0.0004. p =0.0015. p =0.03. p =0.028. p=0.0434. 0. 1.0. 0. 1.0. PU-H71 (µM). 0. 1.0. 0. 1.0. PU-H71 (µM). 0. 1.0. 0. 1.0. PU-H71 (µM). C. A549. MDA-MB-231.

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Insoluble

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  1. S.2 A B MDA-MB-231 PaTu2 MDA-MB-231 e.v. PRKD2-wt e.v. PRKD2-wt e.v. PRKD2-wt p=0.0004 p=0.0015 p=0.03 p=0.028 p=0.0434 0 1.0 0 1.0 PU-H71 (µM) 0 1.0 0 1.0 PU-H71 (µM) 0 1.0 0 1.0 PU-H71 (µM) C A549 MDA-MB-231 Time (hours) Time (hours) PRKD2 PRKD2 Control Control MDA-MB-231 A549 b-actin b-actin Insoluble PRKD2 PRKD2 PRKD2 PU-H71 PU-H71 PRKD2 Soluble b-actin b-actin U 1 2 3 4 5 6 12 24 U 1 2 3 4 5 6 12 24 MDA-MB-231 A549 NH4Cl (20 mM) - + - + - + - + Time (hours) Time (hours) - - + + PU-H71 (0.5 µM) - - + + CHX (5µM) CHX (5µM) D PRKD2 Insoluble PRKD2 Soluble MG132 (10 µM) - + - + - + - + - - + + PU-H71 (0.5 µM) - - + + E

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