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S-figure 1 S-Fig. 1 Measurement of laccase activity in transplastomic lines. 30 µg total soluble protein from leave in 20 mM sodium acetate buffer (pH 5.0) was used. Laccase activity was determined using a spectrophotometer at 420 nm with 2 mg/ml of 2,2.-azinobis-(3-ethylbenzthiazoline-6-sulphonate). Data are expressed as mean ± SD (n=3). vc, vector control; L-2 and L-6, laccase-expressing line 2 and 6.