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Molecular Module

Molecular Module. RNAP. s 70. RNAP. s 32. DnaK. mRNA( s 32 ). mRNA( s 70 ). s 32. s 70. RNAP. mRNA (RNAP). The snaps indicate molecular interactions. HEAT. HEAT. mRNA( s 32 ). Pun. Pfold. s 32. mRNA (Pfold). Pun. Pfold. s 32. s 32 :DnaK. Pun. DnaK. s 32. FtsH. DnaK.

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Molecular Module

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  1. Molecular Module RNAP s70 RNAP s32 DnaK mRNA(s32) mRNA(s70) s32 s70 RNAP mRNA (RNAP) The snaps indicate molecular interactions.

  2. HEAT HEAT mRNA(s32) Pun Pfold s32 mRNA (Pfold) Pun Pfold s32 s32:DnaK Pun DnaK s32 FtsH DnaK DnaK Pfold mRNA (DnaK) mRNA (FtsH)

  3. Pfold s32 s70 mRNA (Pfold) mRNA (s32) mRNA (s70) gene(Pfold) gene(s32) Promoter gene(s70) Promoter Promoter DnaK FtsH mRNA (DnaK) mRNA (FtsH) gene(DnaK) Promoter Promoter gene(FtsH)

  4. RNAP: s70 RNAP: s70 RNAP: s70 gene(s32) Promoter gene(Pfold) Promoter gene(s32) Promoter RNAP: s32 RNAP: s32 Promoter gene(FtsH) Promoter gene(DnaK)

  5. RNAP: s70 RNAP: s70 RNAP: s70 mRNA (s70 ) mRNA (Pfold) mRNA (s32 ) gene(Pfold) Promoter gene(s70) Promoter Promoter gene(s32) RNAP: s32 RNAP: s32 mRNA (DnaK) mRNA (FtsH) gene(DnaK) Promoter Promoter gene(FtsH)

  6. RNAP: s70 RNAP: s70 RNAP: s70 mRNA (s70 ) mRNA (s32 ) mRNA (Pfold) RNAP: s70:Promoter RNAP: s70:Promoter RNAP: s70:Promoter gene(Pfold) Promoter Promoter gene(s32) Promoter gene(s70) RNAP: s32 RNAP: s32 mRNA (DnaK) mRNA (FtsH) RNAP: s32:Promoter RNAP: s32:Promoter gene(DnaK) Promoter Promoter gene(FtsH)

  7. s32 RNAP mRNA (RNAP) RNAP s70 s32 DnaK s70 mRNA(s70) mRNA(s32) RNAP Functional Module Computer module The CADLIVE graphical notation is employed. The black circle means the binding complex of the components indicated with the arrows.

  8. RNAP DnaK mRNA(s32) Pun s32 Pfold mRNA (DnaK) FB sensor module s32 DnaK

  9. DnaK RNAP HEAT mRNA(s32) s32 gene(s32) Promoter mRNA(s32) FF sensor module s32

  10. s32 Pun DnaK Pfold DnaK RNAP: s32 mRNA (DnaK) Promoter gene(DnaK) mRNA (DnaK) Promoter gene(DnaK) Promoter gene(DnaK) Actuator module for DnaK synthesis mRNA (DnaK) RNAP: s32

  11. mRNA (Lon) FtsH s32 RNAP: s32 mRNA (FtsH) s32:DnaK Promoter gene(FtsH) DnaK Promoter gene(FtsH) Actuator module for FtsH synthesis FtsH mRNA (FtsH) RNAP: s32 Promoter gene(FtsH)

  12. HEAT DnaK mRNA (Pfold) Pfold Pun DnaK Plant module Pun Pfold

  13. Flux Module HEAT Pun s70 RNAP s32 DnaK DnaK Pun Pun Pfold mRNA (RNAP) mRNA(s32) mRNA(s70) mRNA (DnaK) Pfold Pfold s32 DnaK mRNA (DnaK) RNAP: s32 DnaK RNAP s32 s70 mRNA (DnaK) mRNA (RNAP) mRNA(s32) mRNA(s70) Promoter gene(DnaK) Plant module SEQ-FB Flux Module (DnaK) Computer module A flux module is constructed by connecting the respective functional modules, where some molecular modules are overlapped. DnaK Actuator module for DnaK synthesis FB sensor module

  14. mRNA (RNAP) mRNA(s70) DnaK s70 RNAP s32 FtsH mRNA(s32) s32 mRNA(s70) s70 Computer module DEG-FB Flux Module (FtsH) s32:DnaK FtsH RNAP: s32 mRNA (FtsH) DnaK Actuator module for FtsH synthesis Promoter gene(FtsH)

  15. HEAT mRNA(s32) RNAP s32 DnaK RNAP s70 s32 mRNA (RNAP) mRNA(s32) mRNA(s70) FF FF Flux ModuleFeedforward FF sensor module Computer module

  16. s32 Amplifier Flux Module s32:DnaK DnaK mRNA (RNAP) mRNA(s70) FtsH Amplifier s32 s70 RNAP HEAT RNAP: s70 mRNA (s32) Promoter gene(s32)

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