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BIO 316 CELL BIOLOGY Dr. Karen Schmeichel November 4, 2008 Lecture # 18 Microtubules, MT Motors & Cell Adhesion. Returned materials Extra Credit Assignment: 5 pts>OUMA 10 pts >> Connect Bio to OUMA Today’s Lab:BB’s JC & Project brainstorming LA#4 Due 11/11

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  1. BIO 316 CELL BIOLOGYDr. Karen SchmeichelNovember 4, 2008Lecture # 18Microtubules, MT Motors& Cell Adhesion

  2. Returned materials • Extra Credit Assignment: • 5 pts>OUMA • 10 pts >> Connect Bio • to OUMA • Today’s Lab:BB’s JC & • Project brainstorming • LA#4 Due 11/11 • Project Proposal/Shopping • Lists Due 11/11 Business:

  3. Objectives: • Discuss MT structure, dynamics and organization within cells • Understand MT-based motors and the work they perform • Describe how cytoskeletal elements • interact with plasma membrane to • allow for cell-cell and cell-substrate • adhesion

  4. Fig. 18-1 CSK Overview

  5. MTs are Essential to Numerous Cell Events

  6. Fig. 18-2 Stable MTs: Cilia in Oviduct

  7. Fig. 18-2 More Stable MTs: Neuronal Axons

  8. Dynamic MTs: Mitotic Spindle

  9. Fig. 18-3 MTs Comprised of / Tubulin Subunits

  10. Fig. 18-3 / Tubulin Subunits Form a Tube (+) (-)  Tubulin Is Exposed on the (-) end

  11. Fig. 18-4 Various MT Arrangements

  12. Fig. 18-5 MTOCs = Centrosomes

  13. Fig. 18-6 Structure of Centrosomes

  14. Cilia: A Closer Look

  15. Evolution of Multicellularity a la Volvox 1632-1723 Gonium Pandorina Volvox

  16. Microtubule Temperature-Dependent Assembly and Disassembly

  17. Three Stages of MT Assembly

  18. Fig. 18-9 Visualizing Microtubule Polarity

  19. Fig. 18-11 MTs and Dynamic Instability Movie at www.whfreeman.com/lodish6e -- Chapter 18 “Cytoplasmic assembly of microtubules in cultured cells”

  20. Fig. 18-10 MTs Disassemble Occurs Via Rapid Catastrophe

  21. MTs and Dynamic Instability

  22. Drugs That Alter MT function Taxol : Stabilizes MTs Colchicine: Destabilizes MTs Both Used as anti-cancer drugs

  23. Fig. 18-13 MTs grow from the MTOC

  24. Linear MT-Based Motors: Kinesins & Dyneins

  25. Microtubule Motors

  26. Fig. 18-18 Motility Along Squid Giant Axon

  27. Fig 18-27 Organelle Transport

  28. MT-based Fish Pigmentation: http://www.borisylab.northwestern.edu/ pages/supplemental/actin.html

  29. Fig 18-41 MT-based Spindle Mechanics

  30. Conclusions for Microtubules: • Largest of the cytoskeletal filaments • / tubulin heterodimers are organized into protofilaments that align laterally to make a cylinder or tube-like structure • MTs display intrinsic polarity with the (+)-end being the faster growing end • Many MTs are organized in cells wrt to the • MTOC/Basal Body • MTs facilitate cargo transport via the action of molecular motors (kinesins, dyneins etc.)

  31. For Next Lecture: • Reading: Ch 19: 801-839 • & Vascular Disease Case Study

  32. Fig. 19-1 Cellular Adhesive Interactions

  33. CELL ADHESION

  34. Fig. 19-15 Tight Junctions Perform a Barrier Function

  35. Fig. 19-16 TJs Prevent Passage of Large Molecules Through Extracellular Space

  36. Fig. 19-9 Adherens Junctions Lie Just Beneath the TJ

  37. Fig. 19-12 Protein Constituents of Adherens Junctions

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