1 / 7

Lab 23

Lab 23. Respiratory Microorganisms. Objectives. Interpret hemolysis results Classify various Streptococcus species. Streptococci. There are 19 groups (A-R) of Streptococci that are classified based on their characteristics Some aggressively lyse RBC Some hydrolyse esculin

albert
Download Presentation

Lab 23

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Lab 23 Respiratory Microorganisms

  2. Objectives • Interpret hemolysis results • Classify various Streptococcus species

  3. Streptococci • There are 19 groups (A-R) of Streptococci that are classified based on their characteristics • Some aggressively lyse RBC • Some hydrolyseesculin • Some are highly salt tolerant • Some are catalase positve

  4. Sheep Blood Agar • 5% Sheep whole blood added to TSA base • Hugely nutritious media • Complete or partial hemolysis (α, β) • Some judgment calls • Look for clearing

  5. For today… • No barfing • We want throat cultures, not tongue cultures • Beware the gag reflex • 1 SBA Plate per pair • Wet sterile swab and go for the sides or back of the throat (carefully) • Inoculate the 1st region and streak for isolation using the loop. • You need isolated colonies for use in the next 2 labs! • Incubate in candle jar, not aerobically

  6. 2nd session • Interpret hemolysis (look through the plate) • Yeast and other organisms can cause β hemolysis • Gram stain 2-4 colonies (if possible choose a representative α and β) • If you have G+ cocci (you should) • Inoculate new SBA (Plus bacitracin disc) • Bile esculin slant • Salt tube

  7. 3rd Session • Measure zone of inhibition • Clearing doesn’t necessarily mean susceptibility • Catalase and Oxidase test colonies off SBA or Bile Esculin slants. • Compare with figure 23.1 (pg 190)

More Related