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Liverpool, 17-21 February 2003 INRA, Avignon, France V. Chovelon, research scientist

Garlic and Health, P9 report. Liverpool, 17-21 February 2003 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician F. Mirrione, temporary agent (1.5 month). Objectives. Somatic embryogenesis, genetic characterisation plant regeneration

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Liverpool, 17-21 February 2003 INRA, Avignon, France V. Chovelon, research scientist

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  1. Garlic and Health, P9 report Liverpool, 17-21 February 2003 • INRA, Avignon, France • V. Chovelon, research scientist • JP Leroux, research technician • F. Mirrione, temporary agent (1.5 month)

  2. Objectives • Somatic embryogenesis, genetic characterisation • plant regeneration • acclimatisation and field evaluation • sanitary aspects • meristem culture on selected clones • production of virus-free bulbs

  3. Milestones • Embryogenesis

  4. Milestones • Sanitary aspects

  5. Deliverables

  6. Rouge Reunion • plants from callus and cell suspensions • In vitro culture (1) • acclimatisation (2) • AFLP analyses • flow cytometry • phenotypic notations - 1st field evaluation at Reunion Island by P8 (3)

  7. Messidrôme and Morasol plants from callus or cell suspensions • In vitro culture (2) - regeneration - bulb maturation • Acclimatisation (2) • 1st field culture (3) (4) 166 Mes cal; 145 Mol cal; 125 Mol ecs;153 Mes ecs • 2nd field culture(4) - 282 Messidrôme cal - 143 Morasol cal - 145 Morasol ecs

  8. 1st field evaluation • notations (plant development, height, leaves colour, bulbs shape and weight) • ploïdy level

  9. 1st field evaluation • sulphur compounds concentration (HPLC) Morasol and Messidrôme Rouge Reunion

  10. 1st field evaluation • sulphur compounds proportion (HPLC) Morasol and Messidrôme Rouge Reunion

  11. Printanor plants from callus

  12. Sanitary aspects • production of virus-free plants • Messidrôme, Morasol, Printanor • 300 plants / var. (Nov-Dec 02) • virological control • all the plants were potyvirus-free • some one contained latent viruses • Messidrôme: 100% GCLV • Morasol: 83% MbFV • Printanor: 100% MbFV

  13. Sanitary aspects • virus elimination by meristem culture

  14. Workplan for 2003 • Embryogenesis, genetic characterisation • Rouge Reunion • 2nd field evaluation (P8), biochemistry (P5) • Messidrôme, Morasol and Printanor from callus • 2nd field culture and evaluation (P9) • flow cytometry (P8), AFLP (P1), biochemistry (P5) • Messidrôme, Morasol and Printanor from cell suspension • 1st field evaluation • flow cytometry (P8), AFLP (P1), biochemistry (P5) • Sanitary aspects • production of virus-free bulbs (P9)

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