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Wellesbourne, 4-5 October 2001 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician C. C

Garlic and Health, P9 report. Wellesbourne, 4-5 October 2001 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician C. Caron, temporary agent (May 01). The objectives. Somatic embryogenesis plant regeneration and acclimatisation culture and evaluation

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Wellesbourne, 4-5 October 2001 INRA, Avignon, France V. Chovelon, research scientist JP Leroux, research technician C. C

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  1. Garlic and Health, P9 report Wellesbourne, 4-5 October 2001 • INRA, Avignon, France • V. Chovelon, research scientist • JP Leroux, research technician • C. Caron, temporary agent (May 01)

  2. The objectives • Somatic embryogenesis • plant regeneration and acclimatisation • culture and evaluation • sanitary aspects • production of virus-free bulbs • meristem culture

  3. Somatic embryogenesis • milestones • Rouge Reunion, Messidrôme, Morasol: plants from calli and cell suspensions • in vitro culture, acclimatisation • field culture, evaluation • flow cytometry (P8), AFLP analyses (P1), • biochemistry (P5) • morphological and physiological notations (P8,P9) • deliverables • paper on regeneration of garlic plants issued from embryogenic calli

  4. Rouge Reunion • plants from calli and cell suspensions • culture in tunnel • AFLP analyses (P1) • flow cytometry • phenotypic notations • harvest at maturity - field evaluation at Reunion Island (P8)

  5. in vitro development Morasol • plants issued from calli

  6. Messidrôme in vitro development • plants issued from calli

  7. Acclimatisation • Morasol, Messidrôme plants from calli

  8. Messidrôme, Morasol • plants issued from cell suspensions in vitro development

  9. Sanitary aspects • production of virus-free plants • 300 plants/variety • Messidrôme, Morasol • Printanor, Reunion • virological control • Messidrôme: 100% GCLV, 34% MbFV • Morasol: 5.6% GCLV, 83% MbFV • Printanor: 100% MbFV

  10. Sanitary aspects • virus elimination by meristem culture

  11. Workplan for 2001-02 • embryogenesis, genetic characterisation • Rouge Reunion • field evaluation (P8), biochemistry (P5) • Messidrôme and Morasol from calli • culture (P9), flow cytometry (P8), AFLP analyses (P1) • Messidrôme and Morasol from cell suspensions • in vitro culture (P9), acclimatisation(P9) • Printanor from calli and cell suspension • in vitro culture(P9) • Sanitary aspects • production of virus-free bulbs (P9) • meristem culture (P9) acclimatisation, culture

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