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PICODIV Cultures of Eukaryotes Overview of novel cultures for focus during year 2

PICODIV Cultures of Eukaryotes Overview of novel cultures for focus during year 2. Outline. Cultures for major focus year 2 picoplanktonic novel Cultures that may be interesting nanoplankton uncharacterized picoplankton heterotrophs in mixture What are we missing ?. Priority.

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PICODIV Cultures of Eukaryotes Overview of novel cultures for focus during year 2

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  1. PICODIV Cultures of Eukaryotes Overview of novel cultures for focus during year 2

  2. Outline • Cultures for major focus year 2 • picoplanktonic • novel • Cultures that may be interesting • nanoplankton • uncharacterized picoplankton • heterotrophs in mixture • What are we missing ?

  3. Priority

  4. Prasinophyceae

  5. Ostreococcus group

  6. RCC 287 NOUM 15 Also two environmental clones from Roscoff RA001219

  7. RCC 343 PROSOPE_5

  8. Trebouxiophyceae

  9. Nannochloris group RCC 115 RCC 236 RCC 347

  10. Chlorarachniophyceae

  11. RCC 365 PROSOPE_99 • chl b • 9 cis neoxanthine • beta carotene • violaxanthine

  12. RCC 337 RCC 376 PROSOPE_153_1 PROSOPE_97_2 RFLP + pigments Pigments

  13. Stramenopiles

  14. Bolidophyceae

  15. RCC 355 Roscoff RA0004-1 Bolidophyceae ? • Contains fuco, 19’BF and HF

  16. Pelagophyceae

  17. RCC 341 PROSOPE_63 Pelagophyceae ? • Contains fuco, 19’BF and HF

  18. Eustigmatophyceae

  19. RCC 357 RA000711-23-2 Also RCC 353RA00071-23 Nannochloropsis oculata ? • no chl c • beta carotene • violaxanthine

  20. Dictyochophyceae

  21. Villf I 5m RCC 304

  22. Other cultures of interest1 - nanoplankton

  23. Villf I 50m Dinophyceae ? RCC 303

  24. Chlorophyceae RCC 300 Taka 9804 Chlamydomonas noctigama ?

  25. Other cultures of interest2 - uncharacterized

  26. RCC 383 PROSOPE 50-1 Contains fuco and 19’ HF

  27. RCC 377Chrysamoeba ? PROSOPE 96-1

  28. RCC 384 PROSOPE 137 Contains fuco and 19’ HF

  29. RCC 333 RCC 388 PROSOPE 114_2 PROSOPE 96

  30. Other cultures of interest3 - heterotrophs

  31. Paraphysomonas is a very common contaminant • Cafeteria sp. -> RCC 334 and 351 • Thraustochytrid -> RCC 194 • LKM 74 -> RCC 358 • Stramenopile -> RCC 363 (mix of 2 auto + 1 het) • -> RCC 353 • Alveolata group II -> RCC 366 (Chl b) • Ciliate -> RCC 82

  32. What are we missing ? • Prasinophyceae • Mantoniella (coastal) • environmental clones • Cryptophyceae • Prymnesiophyceae • environmental clones • Bolidophyceae • coastal strains

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