1 / 14

Laboratory Procedure Courses National Network of Prevention Training Centers

Laboratory Procedure Courses National Network of Prevention Training Centers. Linda Creegan, MS, FNP California STD/HIV Prevention Training Center Oakland, CA National STD Prevention Conference Chicago, March 2008. CLINICIANS…. Are you new to the STD clinic?

amandla
Download Presentation

Laboratory Procedure Courses National Network of Prevention Training Centers

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Laboratory Procedure CoursesNational Network of Prevention Training Centers Linda Creegan, MS, FNP California STD/HIV Prevention Training Center Oakland, CA National STD Prevention Conference Chicago, March 2008

  2. CLINICIANS….. Are you new to the STD clinic? Is your clinic starting to use a new test? Trichomoniasis? Herpes? Bacterial Vaginosis? Syphilis? Rapid HIV test? Darkfield microscopy?

  3. Enroll in an NNPTC laboratory procedure course!! • Tests for STD specialty clinics • Syphilis tests • Gram stains • Saline and KOH wet mounts • Point-of-Care (POC) tests for STD

  4. Tests for STD Specialty Clinics

  5. Syphilis Tests • Serologic tests for syphilis • Learn to perform and interpret the qualitative RPR test • Darkfield microscopy • Learn to • Obtain the specimen • Use the microscope • Identify T. pallidum by morphology and motility

  6. Utility of Rapid RPR Test • Evaluation of patients withsymptoms of primary and secondary syphilis • Facilitates immediate diagnosis, treatment, and initiation of partner management activities

  7. Darkfield Microscopy Photo credit: Dr. Joseph Engelman, San Francisco City Clinic

  8. Morphology Rigid, uniform, tightly-wound spirals Tight coil is maintained despite active motility Length: 6-20 micrometers Width: VERY thin 0.10-0.18 micrometers, below the resolution of the light microscope Motility Exhibits three types of movement Translation: slow, forward or backwards movement Rotation: turning around the longitudinal axis Flexion: stiff bending, usually in the middle, and returning to original position Identifying T. pallidum by DF

  9. Gram Stains • Male urethral discharge, cervical and rectal specimens • Learn to • Obtain the specimen • Identify WBCs and GNID • Diagnose gonococcal vs non-gonococcal urethritis STD Atlas, 1997

  10. Saline and KOH Wet Mounts Vaginal specimens • Learn to • Obtain the specimen • Use the microscope • Identify findings (Clue cells, yeast forms, trichomonads) • Diagnose BV, yeast, trich Photo credit: Seattle STD/HIV Prevention Training Center

  11. Point-of-Care Tests for STD

  12. What are Point-of-Care tests? • Rapid diagnostic tests • Run in clinic or field setting • Very easy to perform • Usually require only microscope or simple lab equipment • Often CLIA waived • Provide results at same clinic visit • Diagnostic and screening applications

  13. Point-of-Care STD Tests:A Paradox • Generally not as sensitive as delayed lab tests • In populations where patients are less likely to return for results or F/U is difficult, higher treatment levels can be achieved with POC than with delayed tests • Homeless clinics • Emergency rooms • Correctional facilities

  14. Point-of-Care Test Courses • Learn about • Lab regulatory issues (CLIA) • POC tests for genital herpes, vaginitis, syphilis, CT and GC, and HIV • Obtaining specimens • Using test kits • Interpreting results • Maintaining quality assurance

More Related