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PCR Team

PCR Team. Thermocycler and Fluid Delivery System. Outline. Background Budget Schedule Thermocycler CFP Fluid Delivery CFP Conclusion Contact Information. Background. Purpose Develop a marketable device to amplify DNA through a process called PCR Benefits

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PCR Team

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  1. PCR Team Thermocycler and Fluid Delivery System

  2. Outline • Background • Budget • Schedule • Thermocycler CFP • Fluid Delivery CFP • Conclusion • Contact Information

  3. Background • Purpose • Develop a marketable device to amplify DNA through a process called PCR • Benefits • Improving Personalized Medicine • Analyzing Potential Drug Interactions • Analyzing Genetic Characteristics • Requirements • Fast • Portable • Inexpensive

  4. Background: Current Problems • Fluid Delivery: • Number of Samples • Changing Samples • Thermocycler: • Time to Steady State • Uniform Heat Distribution

  5. Budget

  6. 9/01 10/01 11/15 12/01 1/15 3/01 3/15 4/15 Begin Design Prototypes Build Thermocycler Control System for Thermocycler Build Fluid Delivery System Combine Thermocycler and Fluid Delivery Customer Testing Finished Product Schedule

  7. Thermal CFP: Motivation • Slide needs to be secured against the heater • Slide must not move during testing • Slide needs to have a quick removal and installation mechanism

  8. Thermal CFP: Prototypes • Cam Clamping • Spring Loaded Clamping • Foam Clamping • Silicon Clamping

  9. Thermal CFP: Prototypes Cam Spring Aluminum Plate Adjustment nut Cam CFP

  10. Thermal CFP: Prototypes Aluminumplate Spring Glass Slide Spacer Chamfers Spring CFP

  11. Thermal CFP: Prototypes Silicon Foam Aluminum plates Glass Slide Silicon CFP

  12. Thermal CFP: Prototypes Foam Foam CFP

  13. Thermal CFP: Testing Three testing criteria: • Vibration test • Drop test • Force test

  14. Thermal CFP: Testing of Clamps • VibrationTest Foam CFP Vibration machine

  15. Thermal CFP: Testing of Clamps • Drop Test Spring CFP 6 inches

  16. Thermal CFP: Testing of Clamps • Force Test Custom slide Force gage

  17. Thermal CFP: Testing of Clamps • Based on testing criteria: 1st – Cam 2nd – Silicon 3rd – Foam 4th – Spring

  18. Thermal CFP: Results Chips from corners • Cam • Cracked Slides • Non-uniform clamping • Silicon and foam • Difficult to install slides • Spring loaded • Difficult to install slides • Did not hold slides well Crack Cracked slide from Cam CFP

  19. Thermal CFP: New Clamp Design convection shield • Incorporates both the silicon and the cam. • Cam clamps on base instead of slide • Only silicon is in contact with slide Silicon Modeled by Chad Meeks and Steve Rabe

  20. Thermal CFP: What’s next? • Finalize the solid model and drawings. • Build the device. • Begin controls portion of project.

  21. Fluid CFP: Motivation • Verify pressure drop in microfluidic channels where PCR is performed. • Determine the amount of tubing that will decrease the flow rate to 0.5 and 5 μl/min.

  22. Fluids CFP: Pressure Test What gage pressure is required to push the fluid through the microchannel? 1-1.5 bars Syringe Pressure Gage

  23. Fluid CFP: Decreasing Flow Rate Syringe Pump Coil of Tubing Short Tubing

  24. Fluid CFP: Test Results Third Order Poly-fit: dV/dt = -4.27e-5L3 + 0.97e-3L² - 5.53e-3L + 7.11

  25. Flow Rate (μl/min) 5 0.5 Tubing (in) 60 62.5 Fluid CFP: Test Results

  26. Fluid CFP: What’s Next? • Improve equation relating fluid flow rate to coiled tube length with further testing. • Analyze available pumps for application to the PCR device.

  27. Conclusion • Thermocycler clamping mechanism will combine the cam and silicon prototypes • Analyzing different pumps and the required tubing for flow reduction.

  28. Jenny Greer jennygreer.eng@gmail.com Seth Plaizier splaizier@gmail.com Chad Meeks chky071@yahoo.com Steven Rabe srabe@ybfa.com Dan Torgerson dantorgerson@hotmail.com Christian Sellers csellers82@hotmail.com Contact Information References: Thermal CFP pictures taken by Seth Plaizier Fluid CFP pictures taken by Dan Torgerson

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