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This is a wide field view of a population of nondividing cells. My protein is red. The DNA is in blue…the chromosomes are uncondensed and just hanging out, running the cell. The microtubules make a network around the cell, organized from a spot within the red staining for my protein. That spot is called the microtubule organizing center. Clever name. A better look at the MTOC on the next slide. my protein microtubules DNA
It’s at the Golgi apparatus in interphase (nondividing) cells I think this staining pattern looks like an engagement ring. You can also see little speckles of my protein in the nucleus. This cell is not dividing, it’s just living its little cell life. And you can see the MTOC, the spot where all the green lines seem to start. my protein microtubules DNA
When I wash the cells with soap first, I can see my protein at centrosomes (the microtubule organizing center) …plus this cell looks like the spiders in Spider-Man, hee hee my protein microtubules DNA
The cell on the right is dividing…you can see the chromosomes in a big pile. The green dots are kinetochores, circles of proteins that hug the chromosomes at their middles and also attach to microtubules, allowing them to pull the chromosome pairs apart when the cell divides. This cell has a long way to go before it can divide. The chromosomes have not lined up along the metaphase plate yet.
Another dividing cell…my protein stays at the centrosomes while the microtubules pull on the chromosomes! Each of the microtubules between the yellow dots (red+green= yellow) is attached to one kinetochore and is pulling toward the centrosomes (red). Eventually all of the chromosomes will go to one side or the other and the cells will divide. my protein microtubules DNA