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Forensic Characterization of Semen

Forensic Characterization of Semen. The presence of seminal stains is important in crimes involving sexual offenses. Two steps: Stains must be located Stains are tested to determine identity. Testing for Seminal Stains. Stains can sometimes be identified visually.

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Forensic Characterization of Semen

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  1. Forensic Characterization of Semen • The presence of seminal stains is important in crimes involving sexual offenses. Two steps: • Stains must be located • Stains are tested to determine identity

  2. Testing for Seminal Stains • Stains can sometimes be identified visually. • Acid phospatase test- Acid phosphatase is an enzyme found in large concentrations in semen. 2 tests: • Acid phophatase reacts with sodium alpha naphthylphospate and Fast Blue B dye to give a purple color. • Acid phospatase reacts with 4-methyl umbelliferyl phosphate, which fluoresces under UV light

  3. Testing for Seminal Stains • Microscopic Examination- Semen can be identified by the presence of spermatozoa • Prostate Specific Antigen – antigen/antibody reaction confirms the presence of seminal fluid

  4. Collection of Rape Evidence Evidence of rape is not limited to semen • Physical injuries • Blood • Hairs • fibers

  5. Physical evidence collected from a rape victim include: Pubic combings Pubic hair reference samples External genital dry-skin samples Vaginal swabs Cervix swabs Rectal swabs Oral swabs Head hairs Blood sample Fingernail Scrapings Clothing Urine specimen Collection of Rape Evidence

  6. Collection of Rape Evidence If a suspect is apprehended, the following physical evidence would be collected. • Clothing • Pubic hair combings • Head and pubic hair samples • A penile swab • Blood sample or buccal swab

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