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Denaturation temperatuare gradient-polymerase chain reaction: by-product?

Denaturation temperatuare gradient-polymerase chain reaction: by-product?. Ji Youn Lee Cell and microbial engineering laboratory Seoul National University. Let ’ s talk about DTG-PCR. A newly invented tool by modifying the typical PCR for solving traveling salesman problem

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Denaturation temperatuare gradient-polymerase chain reaction: by-product?

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  1. Denaturation temperatuare gradient-polymerase chain reaction: by-product? Ji Youn Lee Cell and microbial engineering laboratory Seoul National University

  2. Let’s talk about DTG-PCR • A newly invented tool by modifying the typical PCR for solving traveling salesman problem • More amplification of DNA strands with lower melting temperature • Is it True? • Does it operate Well? • Is it Worth?

  3. It’s time to study DTG-PCR • Experimental part • Modeling and simulation part • Make use of the published ones : Competitive PCR • Addition of the ssDNA distribution profile with temperature

  4. Let’s think about it!

  5. Experimental considerations • DNA strands with different Tms • Same length, different Tm • or Different length, different Tm • or Different length, same Tm… etc. • Experiments • Mix the strands (two or more) • Perform DTG-PCR in various conditions • Analysis: TGGE, DGGE or graduate PCR

  6. Theoretical considerations • Studies up to date • Competitive PCR • Quantitative PCR • Internal standards and products • Anal Biochem (1993) • Quantitative PCR: Theoretical considerations with practical implications • Replication efficiency E (product and standard)

  7. NAR (1996) • A mathematical model and a computerized simulation of PCR using complex templates • Probability calculation • PNAS (1996) • Efficiency of DNA replication in the PCR • NAR (1997) • A coalescent approach to the PCR • NAR (1998) • Modeling and analysis of competitive RT–PCR

  8. JTB (1997) • Enzymological considerations for a theoretical description of the quantitative competitive PCR • Michaelis-Menten equation (enzyme kinetics) • JTB (1997) • Theoretical description of the PCR • JTB (1999) • Polymerase chain reaction: Markove process approach • Bioinformatics (2001) • Virtual PCR

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