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Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans

Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans. H.Schröder, A.Heimers, R.Frentzel-Beyme, A.Schott and W.Hoffmann. Radiation Protection Dosimetry, 103:211-219 (2003). Human T-lymphocyte metaphase. Karyotype 46, XY.

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Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans

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  1. Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans H.Schröder, A.Heimers, R.Frentzel-Beyme, A.Schott and W.Hoffmann Radiation Protection Dosimetry, 103:211-219 (2003)

  2. Human T-lymphocyte metaphase Karyotype 46, XY

  3. Chromosome- type aberrations distinguishable cytologically at mitosis centric ring chromosome and acentric fragment dicentric chromosome and acentric fragment Two normal chromosomes

  4. Aberrant metaphase Metaphase with two dicentric chromosomes and two acentric fragments

  5. Exclusion criteria • Previous radiotherapy and/or medical use of cytotoxic drugs • Greater than average exposure to diagnostic medical radiation • Heavy smokers (more than 20 Cigarettes a day over more than 10 years) • Previous work in the nuclear industry or other badgemonitored occupation

  6. 2nd division metaphase with sister chromatid exchanges (SCE)

  7. Results of the chromosome aberration analysis

  8. Result of the chromosome aberration analysis after grouping

  9. Distribution of the observed dicentric chromosomes and centric ring chromosomes

  10. Very rare findings • Multicentric ring chromosome in a tetraploid metaphase, most probably consting of 4 chromosomes • One metaphase with pulverized chromosomal material

  11. Results, summary • Method of chromosome aberration analysis is both sensitive and specific • 5-fold elevation of dic and cR compared to the control (2.5 per 1000 M vs 0.5 per 1000 M) • elevation is highly significant • significant elevation of dic and cR also in 9 of the 19 individual cases • distribution of dic and cR deviates from Poisson • very rare finding of a multi-cR in a tetraploid metaphase and pvz chromosome material • biological half life of dic is 3.5 years, contradicts to an acute radiation event

  12. Results of the SCE-Assay

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