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Hey Ladies, its quiz time!. Boggusrl@email.uc.edu. How Rachel is going to do this. Put up a slide Write down the answer Go over it immediately Repeat many many times 113 slides of adventure await!! Did you remember your party hats????. What type of epithelium is this? (how do you know?).
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Hey Ladies, its quiz time! Boggusrl@email.uc.edu
How Rachel is going to do this • Put up a slide • Write down the answer • Go over it immediately • Repeat many many times • 113 slides of adventure await!! Did you remember your party hats????
Transitional Epithelium • How do you know? • Scalloped appearance • Bi-nucleated cells
Smooth Muscle • How do you know? • Nuclei more centrally located • Seen here in both planes of section • In cross section you can tell its smooth muscle because of the centrally located nuclei but also because some cells do not have the nucleus cut in the plane so look anucleate and also because of the varying thicknesses of the cuts.
Peripheral Nerve • How do you know? • Encased by an epineurium • Wavy/bubbly appearance of the nerve fibers within epineurium REMEMBER: CIRCLE OF WAVY SHIT
Serous Demilune • How did you know? • Because most of it looks like a mucous secreting gland (vacuolated cytoplasm with laterally displaced nuclei) But the crescent shaped rim (hence LUNE=Moon!) is more like a serous gland (basophilic) so it’s a serous demilune
Dense Irregular Connective Tissue • How do you know? • Few nucleated cells - fibroblasts • Lots of collagen arranged random-like • Nuclei located outside of the collagen
Brown (multilocular) fat • How do you know? • Bubbly appearance signifies many fat-filled vesicles • Nuclei still laterally displaced • Remember that you have multilocular fat as a stage of differentiation to regular white fat.
Fibrocartilage • How did you know? • No perichondrium evident • Cells located in lacunae • Remember that fibrocartilage is often continuous with surrounding CT.
Intramembranous Bone Formation • How did you know? • Osteoblasts surrounding osteoid with osteocytes enclosed in the osteoid…
Ciliated pseudostratified columnar epithelium with goblet cells • How did you know? • Because Dr. Michaels drilled this into your brains? • NO! (well ok, probably) but also because you can sort of see that all the cells maintain their connection with the basal lamina and you can clearly see goblet cells and cilia
Cardiac muscle • How did you know? • Centrally located nuclei surrounded by large ring of cytoplasm • For the most part, more uniformly shaped cuts • Compare to smooth muscle cut in cross section—different sizes of section and nuclei not present as often in the cut.
Which of the following are Transmission Electron micrographs? And what are the EMs of? C. D. A. B.
A, C, D • REMEMBER THAT FREEZE FRACTURE EMS ARE VIEWED WITH TRANSMISSION EM! • All of them are cillia • Remember the basal bodies (what is the sydrome when you don’t have dynein arms? • Kartageners syndrome • What was used to obtain image B? • Scanning EM
Glycogen • How did you know • Because that is what glycogen looks like on the EM • Compare to collagen cut in cross section • Remember that glycogen particles aggregate and also that they exist OVER other stuctures, usually SER
Golgi Apparatus • Layered like apperance • Which end is trans/cis?
Smooth Muscle cell • How did you know? • Presence of dense bodies and dense plaques as well as caveolin (flask shaped invaginations of the membrane)
ganglion • How did you know? • Bullseye like appearance of the cells. FRIED EGGS
Osteoclasts • How did you know? • Multinucleated cells • Found in indented areas of bone (know its bone because you can see the osteocytes in lacunae (yellow arrows)
Serous glands • How did you know? • Because it looks glandular and there is still color in the cytoplasm. Compare to mucous secreting glands, which have vacuolated cytoplasm
1) Bone Marrow 2) spongy/cancellous bone spicule • How do you know? • 1) you know it is bone marrow because it is found between trabeculae of bone (bright red) and it is rich in lipid and hematopoetic cells (purple) Ok, so you haven’t had this yet, I’m just preparing you for next block. • 2) you know its cancellous bone because you can see the osteocytes in lacunae
Label A-D ____A__________B___________C____________D______
A) Zone of resting cartilage • B) Zone of proliferation • C) Zone of hypertrophy • D) Zone of calcification • What process was indicated? • Endochondrial bone formation
stereocilia • You know because it branches and it is long • Another clue – stereocilia often in epididymis. You don’t know what this looks like yet but it is like 35148743 small tubes like this. ALWAYS stereocilia here
Loose Irregular Connective Tissue • You know because there is a general lack of nuclei and very sparse and randomly placed collagen
Mast Cells • You can tell because it is purple because of metochromasia • And if you look really closely you can see that it is a granule-filled cell.
Dense Regular Connective Tissue • How did you know? • Wavy appearance of collagen • Few cells (fibroblasts) located outside of the collagen fibers
Node of Ranvier • You know because it is stained for myelin and the indentation place without myelin • What does myelin do? • What forms it?