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principe du "co-patching" (résolution : ~ 50 nm)

principe du "co-patching" (résolution : ~ 50 nm). récepteurs séparés. + anticorps. rassemblement séparé. + anticorps. rassemblement coordonné. récepteurs associés. Double transfected cell. Selected field in the two-channels. Gray images - contrast optimalization.

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principe du "co-patching" (résolution : ~ 50 nm)

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  1. principe du "co-patching"(résolution : ~ 50 nm) récepteurs séparés + anticorps rassemblement séparé + anticorps rassemblement coordonné récepteurs associés

  2. Double transfected cell

  3. Selected field in the two-channels

  4. Gray images - contrast optimalization

  5. Binary image / Erode / Dilate / Watershed filter BLUE

  6. Analysis of the fields

  7. Results of patching • Patches in red : 189 • Patches in green: 155 • Patches red and green :153 • Overlap : 2 x Patches(R+G) / Patches(R) + Patches(G) • Result for this field = 2 x 153 / 189 + 155 = 0.889 • 89 % co-patching

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