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Why is this research important?

Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device.

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Why is this research important?

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  1. Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device AmirAli H. Talasaz, Ashley A. Powell, David E. Huber, James G. Berbee, Kyung-Ho Roh, Wong Yu, Wenzhong Xiao, Mark M. Davis, R. Fabian Pease, Michael N. Mindrinos, Stefanie S. Jeffrey, and Ronald W. Davis PNAS, March 2009 Presentation by Sarah Laskey 18 November 2008

  2. Why is this research important? • Rare cell purification and profiling • Circulating epithelial cells • Intermediate between primary tumor and metastases? • CEpC numbers correlate highly with cancer prognosis • Molecular analysis of CEpCs for personalized treatment

  3. MagSweeper: How it works • Magnetic rods sweep through blood samples pre-labeled with magnetic beads.

  4. MagSweeper: Why it’s the best • Minimal sample preparation • High purification efficiency • Large volume throughput • Individual accessibility keeps cells available for downstream analysis • Cell function remains intact • Gene expression is not affected • Opportunity for further optimization

  5. Capture Efficiency and Purity • Purification of 50 HLA-A2 target cells from a solution containing different amounts of background cells.

  6. Enrichment of CEpCs from Breast Cancer Patients • CEpC counts from 17 women with epithelial breast cancers

  7. Summary • Rare cell purification and CEpCs • Magnetic sweeper device isolates cells tagged with magnetic beads • CEpCs isolated from 100% of cancer blood samples, 0% of healthy samples! • MagSweeper isolates rare cells with high purity and efficiency

  8. Future Work • Molecular characterization of purified CEpCs • MagSweeper device optimization • Change magnetic sweep parameters • Engineer shape of the magnet • Other rare cell purification applications for MagSweeper

  9. Thank you! • Any questions?

  10. MagSweeper cell-trapping profile • Cells trapped by device at bottom of well (E) vs. surface (F)

  11. Change in gene expression due to MagSweeper

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