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A. B. C. MDA-MB-453. MCF-10A. MCF-10A. + 468 CM 24 hr. + 468 CM 6 hr. + 231 CM 0.5 hr. No Tx. No Tx. Alone. + 453. + 468. p-STAT3 (Y705). p-STAT3 (Y705). p-STAT3 (Y705). STAT3. STAT3. STAT3. Actin. GAPDH. GAPDH. D. E. F. MDA-MB-453. MCF-10A.
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A B C MDA-MB-453 MCF-10A MCF-10A + 468 CM 24 hr + 468 CM 6 hr + 231 CM 0.5 hr No Tx No Tx Alone + 453 + 468 p-STAT3 (Y705) p-STAT3 (Y705) p-STAT3 (Y705) STAT3 STAT3 STAT3 Actin GAPDH GAPDH D E F MDA-MB-453 MCF-10A MDA-MB-453 + BCF CM 0.5 hr + 231 CM 24 hr + 231 CM 1 hr + 231 CM 4 hr + 231 CM 6 hr + 231 CM 0.5 hr No Tx No Tx +231 CM 1 hr +231 CM 24 hr +231 CM 48 hr No Tx p-STAT3 (Y705) p-STAT3 (Y705) p-STAT3 (Y705) p-JAK1 STAT3 STAT3 p-JAK2 GAPDH GAPDH GAPDH