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p14 ARF

Supplementary Figure 2. a. b. Ctl. p14 ARF. p14 ARF. Ctl p14 ARF. STAT3. pSTAT3Y705. Bcl2. Tubulin. pSTAT3-Y705. c. Ctl. sip14 ARF. p14 ARF. STAT3. pSTAT3Y705. Bcl2. Tubulin.

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p14 ARF

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  1. Supplementary Figure 2 a b Ctl p14ARF p14ARF Ctl p14ARF STAT3 pSTAT3Y705 Bcl2 Tubulin pSTAT3-Y705 c Ctl sip14ARF p14ARF STAT3 pSTAT3Y705 Bcl2 Tubulin p14ARF promotes accumulation of pSTAT3-Y705 and downregulation of Bcl-2 expression. (a) H3255 cells were transfected with either empty (Ctl) or p14ARF encoding vector. Expression of p14ARF, STAT3, pSTAT3-Y705 and Bcl-2 proteins was studied by western blotting after 6 days of G418 selection. Tubulin was used as a loading control. (b) H3255 cells were transiently transfected with control (Ctl) or p14ARF expression vector for 48 hours. The expression level of pSTAT3-Y705 was evaluated by pSTAT3-Y705 staining in p14ARF-transfected cells followed by FACS analysis. (c) H3255 cells were transfected with control or p14ARF siRNA. Western blotting was performed using indicated antibodies. Tubulin was used as a loading control. Long time exposure was performed for p14ARF immunoblotting in order to verify the efficiency of ARF neutralisation.

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