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Functional RNAs expand the synthetic biological toolbox

Functional RNAs expand the synthetic biological toolbox. Isaacs et al., Nat. Biotechnol . 2006. Engineered translation regulators. Isaacs et al., Nat. Biotechnol . 2004. Translation riboregulator pairs are specific making the technique scalable. Isaacs et al., Nat. Biotechnol . 2004.

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Functional RNAs expand the synthetic biological toolbox

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  1. Functional RNAs expand the synthetic biological toolbox Isaacs et al., Nat. Biotechnol. 2006

  2. Engineered translation regulators Isaacs et al., Nat. Biotechnol. 2004

  3. Translation riboregulator pairs are specific making the technique scalable Isaacs et al., Nat. Biotechnol. 2004

  4. Allosteric riboregulators of gene expression Base-pairing specificity = scalability Bayer and Smolke., Nat. Biotechnol. 2005

  5. Antiswitches are modular and tunable Bayer and Smolke., Nat. Biotechnol. 2005

  6. Riboswitches – Natural Translational Control Tabor et al., Biotech and Gen Eng. Rev, 2006

  7. Riboswitches can regulate via Boolean logic Sudarsan et al., Science 2006

  8. Regulation of protein decay – postranslational control -ssrA proteolysis system Tag sequence: NH3-Protein-AANDENYALAA-COOH Andersen et al., Appl. Environ. Micro., 1998

  9. Standard Assembly (Combining biobricks)

  10. Build a synthetic gene expression system with XOR logic Mayo et al., PLoS Biology, 2006

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