iGEM, The Registry of Standard Biological Parts, And Synthetic Biology

1. iGEM,The Registry of Standard Biological Parts,And Synthetic Biology Randy Rettberg hq@igem.org igem.org Cambridge, UK July 5, 2010

2. Synthetic Biology Question Can simple biological systems be built from standard, interchangeable parts and operated in living cells? Or, is biology so complex that each case is unique?

3. Education Driving Research

4. Device-Level System Diagram 2003

5. Parts- and Device-Level System Diagram

6. iGEM Philosophy iGEM Philosophy: Get and Give Teams are expected to use the parts, ideas, and experience of teams in previous years. Teams are expected to contribute their parts, ideas, and experiences.

7. iGEM 2004 (SBC 04)

8. iGEM 2005

9. iGEM 2005

10. iGEM 2006

11. iGEM 2007

12. iGEM 2008

13. iGEM 2009

15. iGEM 2010 Teams - 2

16. iGEM AND High School Teams • Advisor: Wendell Lim • Advisor: Orion Weiner • Advisor: James Onuffer • Teacher at Lincoln High School: George Cachianes • Teacher at Lincoln High School: Julie Reis • Plus 10 “Buddies”

17. Is iGEM Safe?

18. Is iGEM Secure?

19. iGEM Growth and Scale iGEM Scale and Growth Year Teams Jamboree Total IAP 2004 2005 2006 2007 2008 2009 2010 2011 2012 4 5 13 32 54 84 112 130 180 250 20 70 120 360 570 825 1100 1300 1800 2500 20 70 150 400 750 1180 1650 1950 2700 3700 Or Not !

20. iGEM 2010 and Beyond • 2010 130 Teams, Jamboree at MIT November 6-8 • 2011 180 (?) Teams, • Regional Jamborees in October • World Championship at MIT (Nov. 5-7) • Regional iGEM Headquarters • iGEM Labs and Courses • Sign up at ung.igem.org • iGEM Society, Institution, Foundation • iGEM Alumni Association

21. New Synthetic Biologists

22. Registry Labs

23. Synthetic Biology Courses

24. iGEM Community Now • 7,041 user accounts • 6,513 unique emails • 1,782 users have logged in since Feb 2010 • 920 have entered parts • 12,327 parts entered • 5,166 DNA samples at the Registry • 2,328 reported to work • 1,691 parts sent by last year’s teams • 795 sequence confirmed parts

25. The Registry: Quality Not Quantity Parts in the Registry are believed to be of low quality. • The DNA Sample does not match the part sequence or cannot be transformed. • The Part is not well documented in the Registry. • The Part has not been measured. • Interchangeable parts are not possible. • Lots of parts are junk.

26. Part Quality Parts in the Registry are believed to be of low quality. • The DNA Sample does not match the part sequence or cannot be transformed. • DNA Quality Control Program • The Part is not well documented in the Registry. • Curation, Ratings, Judges, Filtering • The Part has not been measured. • BioFab, Jason’s measurement program, Devices • Interchangeable parts are not possible. • BioLint • Lots of parts are junk • Delete, clean up, hide

27. BBa_K274002 - email

28. BBa_K274002 – Main Page

29. BBa_K274002 – Get This Part

30. BBa_K274002 - Show 2 other locations

31. Extra PstI Site - email

32. DNA Samples: Quality Control Program

33. Sample QC: Digest Gel

34. Sample QC: Antibiotic Test Plate

35. Sample QC: Sequence Analysis

36. Sample QC: Sample Provenance

37. The Registry – Pace of Change

38. May 2003

45. partsregistry.org

46. Example Part

47. Parameters, Categories, . . .

48. Catalog Style 2009

49. Plasmids/Assembly

50. 2010 DNA Distribution 2010 Kit Plates 1-3 • 2010 Plasmid Backbones • pSB1C3 • pSB1T3 • pSB1A3 • -Cut with EcoRI, PstI, and DpnI