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ISOLUTE Flash columns FlashMaster & Purification consumables

ISOLUTE Flash columns FlashMaster & Purification consumables. Matthew Cleeve Argonaut Technologies 1101 Chess Drive, Foster City CA 94404 USA. Key factors. Particle size distribution Column cleanliness Moisture content Open access columns Solid sample loading Column capacity.

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ISOLUTE Flash columns FlashMaster & Purification consumables

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  1. ISOLUTE Flash columnsFlashMaster & Purification consumables Matthew Cleeve Argonaut Technologies 1101 Chess Drive, Foster City CA 94404 USA

  2. Key factors • Particle size distribution • Column cleanliness • Moisture content • Open access columns • Solid sample loading • Column capacity

  3. Particle size distribution (PSD) • Genuine 40 - 70m distribution • Minimal back pressure on system – low maintenance • Even flow across column, minimal channelling • Reproducible flow from column to column • Silica free final fractions

  4. ISOLUTE Flash SI

  5. Particle size distribution ISOLUTE Flash SI

  6. Flow Flow Vs Influence of particle size distribution on flow 1611

  7. Loading capacity of the column 10 g sorbent with wide particle distribution 5 g sorbent with narrow particle size distribution

  8. Vs Dilution effect at elution step

  9. Column cleanliness • Proprietary frit and tube cleaning process • Proprietary sorbent cleaning process • Columns can be used non-conditioned without significant detector issues

  10. Moisture content • Moisture content for all ISOLUTE Flash columns controlled to 7% • Intra and inter batch control ensures reproducible retention characteristics • Chosen moisture content will not increase / decrease

  11. Open access columns • Liquid sample volume unlimited when loading off-line • Solid sample (pre-absorbed onto SI or HM-N) applied directly to the top of the main column

  12. Solid sample loading • Liquid samples pre-adsorbed onto diatomaceous earth support, ISOLUTE HM-N • All compounds coat support material; no retention mechanism • Initial mobile phase “elutes” reaction mixture onto the main column • Pre-concentration effect • Narrower band compared with bulk silica gives improved separation • Increased column column

  13. FRONT FRONT C 1 2 3 4 5 6 7 8 9 10 11 12 C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 No separation with wet loading (polar solvent) Good separation with dry loading Separation of a sample insoluble in non polar solvent – dry vs. wet loading When dry loading (using silica sorbent or diatomaceous earth), ensure that the polar solvent is totally evaporated

  14. Column capacity • Ensure compare “like with like” • ISOLUTE 10g column is actually 12.5g, 25% more capacity • All ISOLUTE columns have sorbent mass as detailed in the description

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