1 / 3

OmpA C-Terminus Library

OmpA C-Terminus Library. 5uL of ligation reaction (4500 !! ng DNA) were transformed into chemically competent BL29 Gold DE3 Cells. uL transformant vs. colony count. Biobricking the Fec System. Construct Features:

aurek
Download Presentation

OmpA C-Terminus Library

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. OmpA C-Terminus Library 5uL of ligation reaction (4500 !! ng DNA) were transformed into chemically competent BL29 Gold DE3 Cells. uL transformant vs. colony count

  2. Biobricking the Fec System • Construct Features: • Swappable FecA - FecA is flanked by Nhe1 and AflII sites to allow the easy mutagenesis and replacement of FecA. • Variable Promoters - each component will be on a separate constitutive promoter. • The optimization of GFP expression using promoters of different strengths is planned.

  3. Biobricking the Fec System • Mutagenesis of Fec promoter to weaken gene expression, providing a range of sensitivity. • Mutagenesis of the Fec promoter to remove FUR repressor binding site, allowing easier assays.

More Related