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Oxygen-Binding affects Bonds to C-terminus

Oxygen-Binding affects Bonds to C-terminus. Hemoglobin Dynamics at C-term of beta-subunit. See: O 2 binds The salt-bridge between His-146 and Asp-94 on the same b -subunit breaks The salt-bridge between the Carboxylate at C-term of b -subunit loses contact with Lys-40 of a -subunit.

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Oxygen-Binding affects Bonds to C-terminus

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  1. Oxygen-Binding affects Bonds to C-terminus Hemoglobin Dynamics at C-term of beta-subunit • See: • O2 binds • The salt-bridge between His-146 and Asp-94 on the same b-subunit breaks • The salt-bridge between the Carboxylate at C-term of b-subunit loses contact with Lys-40 of a-subunit • DON’T See: • Fe moving into plane of heme when O2binds • Helix F and FG loop moving when His-91 (F8) on helix-F moves • H-bond with Tyr-145 on and Val-98 (on FG loop) onb-subunit breaking • The H-bond between the Asp-99 of b-subunit and Tyr-42 of a-subunit breaking • NONE of the comparable changes at the C-term of the a-subunit

  2. Unfolding = Denaturation • Disrupt forces that hold protein in tertiary structure

  3. Protein folding

  4. Facilitated folding: Chaperones (22.4)

  5. Misfolding and disease

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