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Biological Applications of Crystallography

Biological Applications of Crystallography. Joseph D. Ng. University of Alabama in Huntsville May 24, 2014. www.uah.edu/nglab. Crystallography is NOT… …. 34Å. All you need to know…for now. sin a = y/h. h. y. cos a = x/h. tan a = y/x. a. a= tan -1 (y/x). x. Bragg ’ s Law. 72 o.

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Biological Applications of Crystallography

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  1. Biological Applications of Crystallography Joseph D. Ng University of Alabama in Huntsville May 24, 2014 www.uah.edu/nglab

  2. Crystallography is NOT……

  3. 34Å

  4. All you need to know…for now sina = y/h h y cosa = x/h tana = y/x a a= tan-1(y/x) x Bragg’s Law

  5. 72o 2.8mm

  6. Let’s calculate Pitch h y 2q X-ray x x x=50mm Film y=2.8mm 2q= tan-1(y/x) = 3.2 Bragg’s Law d= 27.5Å

  7. 72o P= 34Å 2.8mm cosa = x/h cosa = d/P P=d/cosa

  8. Let’s calculate Radius! y=Rsinq y=Rsin(2px/P) dy/dx=Rcos(2px/P) 2p/P So when x=0 dy/dx =Rcos(0) 2p/P X=0 dy/dx = R2p/P X=0 Tan (90o-a)= R2p/P R = 10Å

  9. 34Å

  10. Hydrogen-bonding patters in the base pairs defined by Watson and Crick

  11. Sample of Crystals Obtained

  12. Advanced Photon Source Argonne National Lab

  13. 2.0 Å

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