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How were the yeast cells extracted?

How were the yeast cells extracted?. Method 1: Quench: -80°C, 80% MeOH Extract: 80% MeOH , 100% Chloroform 30 minutes at room temperature Method 2: Quench: -80°C, 80% MeOH Extract: -80°C, 80% MeOH , 100% Chloroform 30 minutes in a dry ice/ EtOH bath Method 3: Quench: ice-cold dH 2 O

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How were the yeast cells extracted?

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  1. How were the yeast cells extracted? • Method 1: • Quench: -80°C, 80% MeOH • Extract: 80% MeOH, 100% Chloroform 30 minutes at room temperature • Method 2: • Quench: -80°C, 80% MeOH • Extract:-80°C,80% MeOH, 100% Chloroform 30 minutes in a dry ice/EtOH bath • Method 3: • Quench: ice-cold dH2O • Extract: -80°C, 80% MeOH, 100% Chloroform 30 minutes in a dry ice/EtOH bath • Method 4: • Quench: ice-cold PBS • Extract: -80°C, 80% MeOH, 100% Chloroform 30 minutes in a dry ice/EtOH bath

  2. AQUEOUS EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  3. AQUEOUS EXTRACT M1 (Group A Aqueous) methanol trehalose threonine valine glutamine glutarate glycine propylene glycol succinate alanine citrate glutamate lactate carnitine arginine isoleucine acetone leucine lycine acetate serine glutathione UDP-glucose asaparagine isobutyrate tyrosine proline aspartate phenylalanine fumarate NAD+ formate

  4. AQUEOUS EXTRACT formate phenylalanine tyrosine tyrosine UDP-glucose NAD+ NAD+ NAD+ UDP-glucose NAD+ fumarate NAD+ NAD+ NAD+ UDP-glucose

  5. AQUEOUS EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  6. LIPID EXTRACT L1 (Group A Aqueous)

  7. LIPID EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  8. LIPID EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

  9. LIPID EXTRACT Black: Extraction A; Red: Extraction B; Blue: Extraction C; Purple: Extraction D

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