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MTT Assay (Fold change)

MTT Assay (Fold change). MTT Assay (Fold change). - 12,5 25 50 100. GW 4064 µM. - 0,3 3 6,5 10. a. b. MCF-7 TR2. MCF-7 TR2. CDCA µM. c. HER2. β -Actin.

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MTT Assay (Fold change)

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  1. MTT Assay (Foldchange) MTT Assay (Foldchange) - 12,5 25 50 100 GW 4064 µM - 0,3 3 6,5 10 a b MCF-7 TR2 MCF-7 TR2 CDCA µM c HER2 β-Actin - 25 50 CDCA µM Supplemental Figure 2: MTT growth assays in MCF-7 TR2 cells treated with vehicle (-) or increasing doses of CDCA (12,5-25-50-100 µM) (a) or GW4064 (0,3-3-6,5-10 µM) (b) for 7 days.Cell proliferation is expressed as fold change ± SD relative to vehicle treated cells, and is representative of three different experiments each performed in triplicate. (c) MCF-7 TR2 cells were treated for 24h with vehicle (-) or CDCA 25 and 50µM before lysis. Equal amounts of total cellular extract were analyzed for HER2 levels by Western blotting. β-Actin was used as loading control.

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