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Degraded GSNO

GSNO. NONOate. SNAP. GSH. GSNO. p21. 120. GSH + SB. 100. P = 0.004. P = 0.02. P = 0.006. GSNO + SB. 100. 2.5. 90. 2.0. 80. 80. Fold change p21 mRNA. 1.5. p21 mRNA Level (% remaining). Fold Change. 60. 70. 1.0. 40. 60. 0.5. 0. 50. 20. GSNO. SNAP. 40. 0.

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Degraded GSNO

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  1. GSNO NONOate SNAP GSH GSNO p21 120 GSH + SB 100 P = 0.004 P = 0.02 P = 0.006 GSNO + SB 100 2.5 90 2.0 80 80 Fold change p21 mRNA 1.5 p21 mRNA Level (% remaining) Fold Change 60 70 1.0 40 60 0.5 0 50 20 GSNO SNAP 40 0 Control Control Control A B NONOate 0 2 4 0 4 8 12 48 Time (h) Time (h) Degraded NONOate Degraded SNAP Degraded GSNO SB202190 (nM) 0 7.5 12.5 25 p21 PBS GSH GSNO 3.0 GSNO 2.5 2.0 Fold Change 1.5 1.0 0 7.5 12.5 25 0.5 SB202190 (nM) C D

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