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Fabrication of Biosensors and Lab Testings III

Fabrication of Biosensors and Lab Testings III. Jack Nguyen Xiaodong Ma 05/14/2008. For today. Theory/Background, Testings – Jack Result, other two experiments– Xiaodong. OBJECTIVES OF PROJECT: Fabrication of FP Biosensors

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Fabrication of Biosensors and Lab Testings III

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  1. Fabrication of Biosensors and Lab Testings III Jack Nguyen Xiaodong Ma 05/14/2008

  2. For today • Theory/Background, Testings – Jack • Result, other two experiments– Xiaodong

  3. OBJECTIVES OF PROJECT: Fabrication of FP Biosensors Repeated Analyze antigen/antibody (Influenza A/anti-Influenza A ) interactions Test different Techniques / Antigens MATERIALS: Fiber Optic Strands, CTS Machine, Matlab, PMMA/Acetone solution, Protein G’(10µg/ml), 2% BSA in PBS, Influenza A(20µg/ml), Anti-Influenza A Ig G(20µg/ml), Distilled water, 3-aminopropyltriethoxysilane, Acetone, Ethanol, Microscope, Light, Controllable stage

  4. Budget Budget: $0, all materials provided by UML

  5. Team Roles

  6. Schedule Followed

  7. Optical Fiber with PMMA

  8. Principle of FP cavity sensor.

  9. Schematic diagram showing the fringe shift

  10. Schematic graph of biosensor Influenza A Primary Antibody Pro.G poly(methyl 2-methylpropenoate) Optical Fiber

  11. Fabrication of Biosensor

  12. Procedure SENSOR PMMA PBS BSA WATER Pro.G WATER PBS IgG Inf.A PBS WATER WATER PBS DATA

  13. Time Duration • Fabrication FP sensor : 30 Minutes • PMMA: 2 Hours • Pro.G: 1.5 Hours • BSA: 1.5 Hours • IgG: 1.5 Hours • Influ. A: 1.5 Hours • Total PBS: 5 Hours • Total Hours for a typical lab test: 13-16 Hr • When a lab test fails, we start all over with the FP sensor fabrication

  14. Experimental setup Chiller Matlab Program CTS Fiber Biomaterials

  15. Experimental setup Polymer tube Fiber Glass tube

  16. Fringes in the air

  17. Fringes in the PBS

  18. Result I without BSA

  19. Result II without BSA

  20. Result III with BSA

  21. PMMA Sheet

  22. Experimental setup Stage Detector Stage power Connector to CTS Sensor

  23. Result

  24. SiO2 /Si VIS spectrometer

  25. Experimental setup Light Source VIS Spectrometer Detector SiO2/Si

  26. Experimental steps • Wash out the dust of SiO2/Si substrates several times by DI water. • Wash the SiO2/Si substrates by sonication in acetone, ethanol and DI water for 15 min, respetively. • Put the cleaned and dry SiO2/Si substrates in 2% solution of 3-aminopropyltriethoxysilane in acetone (3-aminopropyltriethoxysilane : acetone =1 : 49 by volume ) at room temperature for 30 seconds. • Measure the substrates’ spectra by VIS spectrometer after SiO2/Si substrates dried. • Drop Pr.G’ on SiO2/Si substrates for 1.5 h, wash by DI water 3 times, air-dry and detect its spectra. (For comparison experiment, drop BSA on the substrates first and then wash and detect spectra.) • Drop anti-Inf.AIgG on the surface for 1.5 h, wash by DI water 3 times, air-dry and test its spectra. (For comparison experiment, drop Pr.G’ on the surface with BSA and wash, dry detect spectra.) • Drop Inf.A on the surface for 1.5, wash by DI water 3 times, air-dry and then measure its spectra. • Analyze the spectra of two groups experiment.

  27. Results

  28. Results

  29. Results

  30. Results

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