From Natural Diversity to Better Cultivars 10 January 2013 San Diego, California

1. From Natural Diversityto Better Cultivars 10 January 2013 San Diego, California

2. Direction from Feedback Evaluation of needs and priorities has been ongoing in the current RosBREED project: • Information of trait values from stakeholders(Obj. 1 of RB1) • Information of MAB breeding needs and opportunities from breeders (Obj. 5 of RB1) • Input from Advisory Panel Members(Obj. 5 of RB1)

3. Feedback Received • Industry:Risks and opportunities indicate a need to broaden the scope of traits while making sure that fruit quality is still a top priority • Breeders: • MAB adoption has begun, but breeders need help (access to routine accurate genotyping services, genetic knowledge for other traits, implications of trait locus clusters, and knowledge of genotype × environment interaction)

4. Planning Activities in 2012 • Advisory Panel Members: recommendation to consider a second project • U.S. Rosaceae Executive Committee: participation in conference calls and meetings • U.S. Rosaceae breeders and interested colleagues: written input solicited, involved in planning process • Planning group: developed Logic Model • Planning group: identified scope, desired outcomes, and objectives • Advisory Panel members: provide input on scope, desired outcomes, and objectives TODAY

5. RosBREED’s Long Term Outcomes More rapid availability of new cultivars with genetically superior fruit quality Improved profitability and sustainability of U.S. rosaceous fruit, nut, and floral crops with increased consumption and enjoyment

6. RosBREED Approach Improve the efficiency and successof U.S. Rosaceae crop breeding programsthrough the use of DNA diagnostic tests

7. RosBREED #2

8. Risks mitigated, opportunities realized Post-breeding IMPLEMENTATION Breeding Plant diversity identified to address risks & opportunities Pre-breeding DISCOVERY Wild germplasm Elite germplasm Cultivar release & adoption

9. Objectives Structure Obj. 1. Central breeding support Obj. 2. New trait locus discovery Obj. 3. Pre-breeding Obj. 4. Post-breeding Obj. 5. Communicate project outcomes & impacts

10. Supporting Breeding Decisions Which wild germplasm to use? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? WA2 Which advanced selections to commercialize?

11. Obj. 1: Central Breeding Support Goal: Expand support tools Rosaceae-wide for efficient processing of new cultivar-generating plant populations addressing production and market risks and opportunities Success: Valuable & superior new genetic combinations efficiently, accurately, rapidly, and creatively developed and identified; Massive complex breeding data managed efficiently for routine implementation of DNA-informed breeding

12. RosBREED #2

13. Demonstration Breeding Programs

14. Opportunities Addressed • Advances in comparative genomics will allow for more efficient genetic discovery across crops • A new statistical approach (Genome-wide selection) has potential to • increase the precision of • marker-assisted breeding • Advances by international groups/projectswill be leveraged

15. Challenges Addressed • Access to accurate, cost effective genotyping services • Database resources and tools to support MAB • Polyploidy: definingfunctional alleles & • allele dosage

16. Obj. 2: New trait locus discovery Goal: Ensure known trait loci are available for high-priority traits addressing production and market risks and opportunities Success: All U.S. Rosaceae breeding programs have access to promising known trait loci addressing high value traits

17. Supporting Pre-Breeding Decisions Which wild germplasm to use? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? WA2 Which advanced selections to commercialize?

18. Obj. 3: Pre-breeding Goal: Provide access to genepools of wild and novel germplasm for rosaceous crop breeders Success: Valuable alleles from wild or novel germplasm are routinely identified and incorporated into cultivar-generating populations more efficiently, accurately, rapidly and creatively

19. Supporting Post-Breeding Decisions Which wild germplasm to use? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? WA2 Which advanced selections to commercialize?

20. Obj. 4: Post-breeding Goal: Enable and optimize use of DNA information for elite selection advancement and cultivar adoption decisions Success: Breeders and industry sectors will make confident elite selection advancement and cultivar adoption decisions, thereby mitigating risks and realizing opportunities

21. Obj. 5: Communicate project outcomes and impacts Goal: Communicated project outcomes and impacts to all stakeholders Success: Breeders, allied scientists, industry sectors, and the public have an increased understanding of how genetics knowledge is being used to more efficiently and rapidly develop and commercialized new rosaceous cultivars that provide increased consumption, enjoyment, profitability, and sustainability

22. U.S.-Wide and Multi-Disciplinary

23. International Collaborators Plant Research International, Netherlands East Malling Research, UK CRA-FRU, Rome, Italy IASMA, Trento, Italy INRA (Bordeaux, Avignon, Angers), France Andres Bello University, Chile Plant & Food Research, New Zealand ARC, South Africa RosBREED participant location + 2 Scientific Advisory Panel members (Spain, New Zealand)

24. Acknowledgement Contributors to Logic Model Cameron PeaceAmy Iezzoni Robert Shupp Nahla Bassil Sook Jung Eric van de Weg Rex Bernardo Jim Luby Gayle Volk Michael Coe Dorrie Main Vance Whitaker Tom Davis Chris MaliepaardChengyan Yue Kate Evans Vicki McCracken Gennaro Fazio Jim McFerson Karina Gallardo Jay Norelli KsenijaGasic Mercy Olmstead Craig Hardner Brad Rickard

25. Advisory Panel Members RosBREED I First Annual Meeting, 7-8 Jan 2010 RosBREED II Annual Advisory Panel Meeting, 13 Jan 2011

26. Thank you