Federal Centre of Breeding Research on Cultivated Plants

1. Federal Centre of Breeding Research on Cultivated Plants • Introduction of the work plan, timetable of deliverables • Christoph U.Germeier

2. Introduction to the project Project partners and co-sponsors Work packages Milestones and Deliverables in 2007/2008 some questions

3. Project Partners 15 partners from 9 countries P0: Federal Centre of Breeding Research on Cultivated Plants (BAZ), RCC Plant Genetic Resources, Quedlinburg, Germany P1: Plant Breeding and Acclimatization Institute (IHAR), Radzikov, Poland P2: Svalöf Weibull AB Cereal Breeding Department, Svalöv Sweden P3: Federal Centre of Breeding Research on Cultivated Plants, Groß Lüsewitz, Germany P4: eurofins Analytik GmBH| Wiertz-Eggert-Jörissen, Hamburg, Germany P5: INRA Cereal Genetic Resources Centre, Clermont Ferrand, France P6: Institute for Cereal Research of Bergamo, Bergamo, Italy P7: Swedish University of Agricultural Sciences, Department of Food Science, Uppsala, Sweden P8: Experimental Institute for Cereal Research of Fiorenzuola d’Arda, Fiorenzuola d’Arda, Italy P 9: Suceava Genebank, Suceava, Romania

4. Project Partners 14 partners from 9 countries P10: IPGR “K.Malkov”, Sadovo, Bulgaria P11: Jõgeva Plant Breeding Institute, Jögeva, Estonia P12: IHAR, Laboratory of Quality Evaluation of Plant Materials, Radzikow, Poland P14: Agricultural Research Institute Kromeriz, Ltd., Kromeriz Czech Republic P15: Agrotest Fyto, Ltd., Kromeriz Czech Republic 3 Co-sponsors Peter Koelln KGaA, Elmshorn, Germany Emco Ltd., Praha, Czech Republic Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V. (GFP), Bonn, Germany

5. Workpackages and Time Frame 10 Work packages Leader PM Duration 1 Project Management and Coordination P0 (DEU) 8 1 - 48 2 Project working collection P1 (POL) 35 1 - 24 3 Field experiments and sampling seeds for quality analysis P9 (ROM) 93 15 - 40 4 Field experiments with artificial Fusarium inoculation P3 (DEU) 35 15 - 42 5 Fusarium and mycotoxin analysis P15 (CZE) 32 24 - 48 6 Analysis for protein and fat and minerals P5 (FRA) 24 24 - 46 7 Analysis for ß Glucan P6 (ITA) 40 24 - 48 8 Analysis for Antioxidants P7 (SWE) 41 24 - 48 9 Analysis for cold tolerance P8 (ITA) 11 15 - 47 10 Project documentation and internet portal P0 (DEU) 15 1 - 48

6. WP2: Working Collection P1 P5 P0 ELISA PCR P10 P6 P8 P9 Multipliers Provide some germplasm P15 P6 P8 seed seed seed WP4: Fusarium Field Experiments WP9: Frost Tolerance WP3: Quality Field Experiments WP5: Mycotoxin - Fusarium Field- Test P3 P14 P9 P8 P2 P1 P5 P8 P9 P10 P6 P9 P0 P0 P7 P3 P5 P8 P1 P11 P8 P9 Nordic (N) E-Europe SE (N) E SW S (S) E S S-Europe SE samples samples NIRS P11 P5 Climate C. samples P8 NP-HPLC Kalibration WP6: Protein and Fat WP1 Coordination P6 Enzyme P11 P9 P2 P6 Protein Fat WP8: Antioxidants AVA FIA Starch Fibre LC-MS/MS P7 WP7: ß-Glucan/Carbohydrates P12 P4 P15 WP10: Data Management P1 P2 P3 P4 P5 P6 P7 P9 P10 P11 P12 P14 P15 P8

7. Milestones and Deliverables in the WPs (2007/2008) WP1: Management and Coordination March 2007 - March 2008 M1.1 First project meeting M1.2, D1.1 First annual report to the Commission March 2008 - March 2009 M1.3 Second project meeting and mid-term review (Quedlinburg), M1.4, D1.2 Second annual report to the Commission Question: Autumn 2008 for next meeting ok?: - End of WP1 (Multiplication of working collection) - After first field experiment year - Before onset of laboratory work

8. Milestones and Deliverables in the WPs (2007/2008) WP2: Project working collection March 2007 - March 2008 M2.1 Accessions selected for use in the project M2.2, M2.3 1st 300 accessions + 10 standards (5 replications) multiplied M2.4, D2.4 Harvest germinability and infection tested and delivered to field partners March 2008 - March 2009 M2.5, M2.6 2nd 300 accessions + 10 standards (5 replications) multiplied M2.7, D2.5 Harvest germinability and infection tested and delivered Questions: - Methodology of seed testing of harvested material - Germination (plate test, soil test?, protocol) - Test of Fusarium infection? (protocol) - Consequences and thresholds?

9. Milestones and Deliverables in the WPs (2007/2008) WP3: Field experiments and sampling seeds for quality analysis March 2007 - March 2008 M3.1 First project meeting, working collection approved, D3.1 List of descriptors agreed on; March 2008 - March 2009 M3.2 First evaluation year field experiments sown (300 accessions + five to ten standards in five replicdations). M3.3 First evaluation year field experiments harvested. M3.4, D3.2 First year samples distributed to analytical labs and observation results delivered to the project documentation. Questions: - Field design; suggestion: augmented block - Standards, how many? - Descriptors (IBPGR, UPOV, other?) - Plot size, sowing density, standardized?

10. Milestones and Deliverables in the WPs (2007/2008) WP3: Descriptors suggested in the proposal - Colour of lemma, - Color of kernel, - Days to heading and to maturity, - Crop height, - Lodging, - Panicle shape, - Number of sterile spikelets, - Occurrence of diseases, - Hull content (partners with dehulling equipment, e.g. P3), - Thousand kernel weight (200-kernel samples in triplicate), - Test weight. Questions: - Methods (protocols) for observations, measurements preferred where possible

11. Milestones and Deliverables in the WPs (2007/2008) WP4: Field experiments with artificial Fusarium inoculation March 2007 - March 2008 M4.1 First project meeting, working collection approved, D4.1 List of descriptors agreed on; March 2008 - March 2009 M4.2 First evaluation year field experiments sown (300 accessions + five to ten standards in five replications). M4.3 First evaluation year field experiments harvested. M4.4, D4.2 First year samples distributed to analytical labs and observation results delivered to the project documentation. Questions: - same as WP3 and additional: - Control treatment (not inoculated)? - Inoculum, inoculation method?

12. Milestones and Deliverables in the WPs (2007/2008) WP4: Descriptors suggested in the proposal - Panicle emergence (heading) date - Leaf index - Crop height - Plot density (number of panicles per m row) - Panicle shape - Panicle length - Number of kernels per spikelet Questions: - Methods (protocols) for observations, measurements prefered where possible

13. Milestones and Deliverables in the WPs (2007/2008) WP5-8: Analytical WPs March 2007 - March 2008 Mx.1 First project meeting, discussion of methods and sample strategy, Dx.1 Approach strategy for sample selection March 2008 - March 2009 Dx.2. Final plan with adjustment based on field results WP6: M 6.1. 300 Avenin patterns from 1st multiplication by P5

14. Milestones and Deliverables in the WPs (2007/2008) WP9: Analysis for cold and drought tolerance Question: starting not before month 24?

15. Milestones and Deliverables in the WPs (2007/2008) WP10: Project documentation and internet portal March 2007 - March 2008 M10.1, D10.1 Accessions for the working collection selected M10.2, D10.1 Project web portal launched M10.3 Module for online input of germination and harvest data developed M10.4 Module for online randomisation of field plans developed March 2008 - March 2009 M10.5, D10.3 Online interface for creating templates for field observation developed M10.6 Refined object model for C+E data developed M10.7 Online upload of C+E data developed

16. Thanks for your attention