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Advanced Filtration System (ATF) for Concentrated Fed-Batch Processes

This introduction provides an overview of an ATF-manufacturing platform for upstream unit operations in cell cultivation. The system combines the benefits of concentrated perfusion and fed-batch processes, allowing for high cell concentration, straightforward feeding control, and concentrated harvest. The CFB process retains protecting proteins and growth factors, resulting in higher viability without the need for a bleed. The constant ATF rate and controlled feeding ensure efficient filtration and optimized cell growth.

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Advanced Filtration System (ATF) for Concentrated Fed-Batch Processes

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  1. An introduction to a novel filtration system ATF-manufacturing Platform Upstream Unit Operations ATF-cellcultivation Concentrated Perfusion Concentrated Fed-Batch Hybrid Processes

  2. Concentrated Fed-Batch (CFB) • Has the benefits of Concentrated Perfusion (CP) and Fed-Batch together • high cell concentration (70-200m/ml) achievable in 2 weeks • Straight-forward feeding control & removal of unwanted metabolites • Concentrated harvest in one identifiedbatch: CHO at 17g/L (and higher) • CFB allows retention of some protecting proteins and growth factors so viability may be higher than CP, removing the need for a bleed and simplifying the process control ATF rate constant at ~0.05-0.1 vv / min (or faster) Feeding controlled by level probe Filtrate pump runs at 0.5-3 vv / day or as required by cell growth UF filter concentrates protein inside reactor

  3. CFB Results – PER.C6 Cells

  4. CFB Results – CHO Cells

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