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Buffer Capacity Lab. Buffer Solutions. A buffer solution is a solution that changes pH only slightly when small amounts of a strong acid or a strong base are added. A buffer contains significant concentrations of both a weak acid and its conjugate base, or
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Buffer Solutions • A buffer solution is a solution that changes pH only slightly when small amounts of a strong acid or a strong base are added. • A buffer contains significant concentrations of both • a weak acid and its conjugate base, or • a weak base and its conjugate acid.
Importance of Buffers • Our blood is buffered to a pH of 7.4 • Buffers are used when pH must be controlled in experiments. • Proteins • Enzymes
Sodium Acetate Buffer • This buffer relies on the dissociation reaction of acetic acid. CH3COOH CH3COO- + H+
Pure water increases in pH by about 5 pH units when the OH– is added, and decreases by about 5 pH units when the H3O+ is added. In contrast, the same amounts of OH– and H3O+ added to a buffer solution barely change the pH.
Buffer Capacity andBuffer Range • There is a limit to the ability of a buffer solution to neutralize added acid or base. • This buffer capacity is reached before either buffer component has been consumed. • As a rule, a buffer is most effective if the concentrations of the buffer acid and its conjugate base are equal or nearly so.