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Enzyme Dependent-Fluorescence Recovery After Photobleaching (ED-FRAP)

Enzyme Dependent-Fluorescence Recovery After Photobleaching (ED-FRAP). ED-FRAP is a method of quantitatively imaging the distribution of enzymatic activity within small samples or cells.

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Enzyme Dependent-Fluorescence Recovery After Photobleaching (ED-FRAP)

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  1. Enzyme Dependent-Fluorescence Recovery After Photobleaching(ED-FRAP) • ED-FRAP is a method of quantitatively imaging the distribution of enzymatic activity within small samples or cells. • The method relies on the selective destruction of a metabolite using a photobleaching pulse and measuring the rate of enzymatically driven recovery of the metabolite.

  2. NADH ED-FRAP is dependent on the presence of enzyme in vitro GDH and NADH NADH Alone Before Bleach Recover

  3. ED-FRAP is Linearly Enzyme Dependant In Vitro NIH/NHLBI/LCE

  4. ED-FRAP and Intact Cardiac Myocytes Bleach Before Recover

  5. Regional ED-FRAP in Intact Cardiac Myocytes NADH Rate Map:Rest Control NADH fluorescence image ED-FRAP NADH Recovery Rate Constant Image Rate Map: DCA - Rest NADH The rate constant map is basically an image of NADH generating Capacity in the cell. Note the prevalence of mitochondrial in this process

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