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ori pVS1

ori pRi. kan r. pTF101.1::Ri. 10.1 kbp. ori RK2 (incP  ). RB. 2x P 35S. TEV enhancer. bar. ori pVS1. Tvsp. pTF101.1. 9.2 kbp. LB. aadA. pgl. ori colE1. ori RK2 (incP  ). pE2759. 30.1 kbp. ori pSa (incW). pTF101.1::BIN19. pTF101.1::UCD2. tet r. 11.0 kbp. 13.0 kbp.

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ori pVS1

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  1. ori pRi kanr pTF101.1::Ri 10.1 kbp ori RK2 (incP) RB 2x P35S TEV enhancer bar ori pVS1 Tvsp pTF101.1 9.2 kbp LB aadA pgl ori colE1 ori RK2 (incP) pE2759 30.1 kbp ori pSa (incW) pTF101.1::BIN19 pTF101.1::UCD2 tetr 11.0 kbp 13.0 kbp picA Supplemental Figure 1. Schematic representation of the various T-DNA binary vectors and integration plasmid used in this study. The parent plasmid was pTF101.1. The region highlighted in blue indicates the T-DNA region plus the aadA gene incorporated into all other plasmids. LB/RB, T-DNA left/right border regions; ori, vegetative origin of replication; 2xP35S, cauliflower mosaic virus double 35S promoter; TEV, tobacco etch virus; bar, phoshinothricin-resistance gene; Tvsp, vsp polyA addition signal; aadA, spectinomycin-resistance gene; tet/kanr, tetracycline/kanamycin resistance gene; pgl/picA, pgl/picA genes from the C58 chromosome. tetr

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