1 / 27

Adipose Stem Cell Center

Adipose Stem Cell Center. Danielle M. Minteer, BS Department of Bioengineering University of Pittsburgh Central Catholic High School 9 April 2013. Outline. Adipose Stem Cell Center Adipose Tissue Bioreactors Peripheral Nerve Regeneration About Me Pitt Freshman Engineering

bevelyn
Download Presentation

Adipose Stem Cell Center

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Adipose Stem Cell Center Danielle M. Minteer, BS Department of Bioengineering University of Pittsburgh Central Catholic High School 9 April 2013

  2. Outline Adipose Stem Cell Center Adipose Tissue Bioreactors Peripheral Nerve Regeneration About Me Pitt Freshman Engineering Bioengineering program @ Pitt Graduate School

  3. Adipose Stem Cell Center ASC Biology Soft Tissue Repair Wound Healing Nerve Regeneration

  4. Adipose Stem Cell Center Clinicians, Surgeons (MDs) Engineers (PhDs, MDs) Molecular Biologists (PhDs, MDs)

  5. Adipose Tissue FAT! Derived from the mesoderm Found in every mammal and some non-mammalian species Maintains energy metabolism Lipid storage Adipose in Tissue Engineering & Regenerative Medicine

  6. ASC Isolation SVF ASCs

  7. Adipose Derived Stem Cells (ASCs) [1] Du Y, et al. Mol Vis. 2010; 16: 2680-9. [2] Marra KG, et al. Methods in Mol Biol. 2011; 702(3): 261-268. [3] Zhang et al., 2000; J Endocrinol. 164:119-128. [4] Kang et al., 2009; Tiss Engr: Pt A. 15(8):2227-2236. • Adipose-derived stem cells (ASCs) • Differentiate into various tissue types [1] • Traditionally grow in static, 2D, flat surfaced, culture flasks [2] • 2D does not allow lipid accumulation • 3D Culture of adipocytes • Ceiling culture [3] • Gels, scaffolds [4] • May be maintained for only short periods of time

  8. Bioreactors [3] Gerlach J, et al. Tiss Engr Part C; 2012; 18(1): 54-61. Image credits: http://www.exomed3d.com/, http://ajpcell.physiology.org/content/287/6/C1527/F4.small.gif, The Bioreactor Group • Bioreactors • 3D, long-term tissue culture [3] • Dynamic culture environment • Provides a more accurate in vitro model of in vivoconditions • Types • Spinner flasks • Rotating Wall Vessel • Perfusion Chambers

  9. Our Bioreactor System • Hollow, porous fibers • Uniform gas & nutrient exchange • Integrated, decentralized oxygenation • Physiological gradients • Negligible shear stress Dynamic perfusion system + pumps pH regulation

  10. Gerlach, et al., 2012 Our Bioreactor System • Established long-term adipose tissue culture model within a 3D hollow fiber based bioreactor [3] [3] Gerlach J, et al. Tiss Engr Part C; 2012; 18(1): 54-61.

  11. Our Bioreactor System

  12. Peripheral Nerve Regeneration Peripheral nervous system vs. Central Trauma, result of other surgical procedures Peripheral nerves will regenerate on own up to ~ 5cm Tissue engineered nerve conduits

  13. Myself: High School • Competitive cheerleading • Gymnastics • Marching band • Concert band • Jazz band • Softball • Outstanding Young Woman program • New Castle High School 2006

  14. Myself: High School • New Castle High School 2006 • 4.0 < GPA < 4.5; Rank 8/350-400 some • SAT’s twice • 800 Verbal, 580 Math, 800 Writing • ACT • 28 • AP Courses • English (5) • Chemistry (4) • Calculus • Economics • US History

  15. Myself: High School • New Castle High School 2006 • Applied to Pitt, Penn State, Northeastern (Boston) • Why I chose Biomedical Engineering • Sounded cool • “Stable” Career • Diverse as a female • Unsure for a very long time

  16. High School: What I would do differently • Take advantage!!! • AP credits • Shadow your interested career • If interested in research: • ROHSS Opportunity (contact: Dr. KaceyMarra) • PJAS, PRSEF, INTEL ISEF • Summer internships/shadowing • Google search & e-mail Pitt/CMU professors • Apply to programs early, leaves room for more scholarship opportunities • Take your entry/placement exams seriously!

  17. Pitt Freshman Engineering • Begin all together as freshmen • Industrial, Electrical, Computer, Mechanical & Materials Science, Civil & Environmental, Chemical& Petroleum, Bio • Classes first year: • Physics I & II • Chemistry for Engineers I & II • Intro to Engineering I & II (computer stuff) • Freshman Engineering Seminars I & II • Math (typically Calculus I & II) • Electives • More info: http://www.engineering.pitt.edu/Freshman/

  18. Pitt Freshman Engineering: Advice Leave breathing room in your schedules, you’ll be thankful for them when the time comes Show up to classes, even if there is no quiz/homework Learn your studying style; do you really understand the material? Always be thinking of questions (whether you ask the professor or not) Take advantage !! Engineering (SPACE) dormitory living Writing Center, Math Assistance Center on Pitt’s campus Social aspects & benefits of being a college student “Plus 3”

  19. BioE @ Pitt • 4 tracks • Biomechanics • Biosignals & imaging (Most flexible, nice for pre-med) • Cellular Engineering • Medical Device Engineering • Intense – you will work! • A lot of teamwork • Career paths of BioEs • Industry/government • Medical School • Law School • Graduate School: MS (Professional, Thesis/Research-based) • Graduate School: PhD

  20. BioE @ Pitt My course schedule

  21. BioE @ Pitt: Wise words of experience • Group work! • The sooner you learn which career path, the bigger advantage you have • Find out what you like/don’t like • It’s okay to switch majors/colleges • REU = Research Experience for Undergrads • Who says you need to graduate in 4 years? • Enjoy being a student/college life • If you get the chance, travel! • Network! It’s all about who you know!!!

  22. Graduate School: BioE PhD Undergrad @ Pitt BioE  Grad school @ Pitt BioE Extension of undergraduate courses, new people More problem solving-based, less memorization Research is understood to be 1st priority in most classes Preliminary/Qualifying Exam (1st year, 2nd year) PhD Thesis Proposal (3rd year, 4th year) PhD Thesis Defense (4th year, 5th year, 6th year) Stipends: ~$15,000-30,000/year

  23. Graduate School: PhD Life What I Do day-to-day Every day is different! I make my own hours Lab = home base Classes, homeworks, TA-ships Enjoying time before “the real world” Most weekends & evenings to myself Traveling

  24. PhD vs. MD MD • Classes 1st 3 years, very strict schedule • Rigorous studying • Step 1, 2, 3, Boards • Residency, then practice • Industry, Academia/Research, Clinical PhD • Every day is different! • I make my own hours • Stipend • Prelim, Proposal, Defense • Post-doc, then job • Industry, Academia

  25. Roads to PhD vs. MD MD • A lot of luck in addition to hard work • Undergraduate grades, research, volunteer, diversity • MCAT • Average age ~24 • Focus on GRADES & MCAT score • Be prepared for application fees! PhD • Know the right people, make connections early • Undergraduate research & experiences, diversity • GRE • Average age ~27 • Focus on grades & research in undergrad

  26. Contact Me Danielle M. Minteer dmm69@pitt.edu Danielle.minteer@gmail.com Biomedical Science Tower 16th Floor 200 Lothrop St

More Related