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Lab Meeting 2: Literature search on how to quantify and measure hypothesis

Lab Meeting 2: Literature search on how to quantify and measure hypothesis. Paige O’Donnell, investigator December 12, 2008. Overview. Slide 1:Question Slide 2:Hypothesis Slide 3:How cardiovascular anomalies are caused Slide 4:Development of zebrafish Slide 5:Testing my hypothesis

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Lab Meeting 2: Literature search on how to quantify and measure hypothesis

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  1. Lab Meeting 2: Literature search on how to quantify and measure hypothesis Paige O’Donnell, investigator December 12, 2008

  2. Overview • Slide 1:Question • Slide 2:Hypothesis • Slide 3:How cardiovascular anomalies are caused • Slide 4:Development of zebrafish • Slide 5:Testing my hypothesis • Slide 6:Continuation of “Testing my hypothesis”

  3. Question • Are the defects that are caused by fluoxentive and paroxetine dependent on the dosage given?

  4. Hypothesis • I hypothesize that the dosage amount will determine the extent of the defects that are caused by the SSRIs, fluoxetine and paroxetine.

  5. Cardiovascular Anomalies • Usually occur within the first ten weeks of a human fetus • Very beginning of development • Present at birth • A two day zebrafish embryonic heart is the equivalent to a 3 week gestation human embryonic heart

  6. Development of zebrafish • It takes about 72 hours for the zebrafish to complete embryonic development • Between the 10 hour period and the 14 hour period the beating heart becomes visible • After 35 hours the zebrafish will break out of its sac and begin to swim about

  7. Testing My Hypothesis • The first part of either proving or disproving my hypothesis is to keep an accurate count of how many eggs there are • As time progresses, the eggs will become fertilized and begin developing • As this occurs it is important to keep track of how many become fertilized and progress, how many are not fertilized, and how many die because of the teratogenic effects of SSRIs

  8. Testing My Hypothesis (continued) • The other aspect of testing my hypothesis will be to monitor the beating heart of the fish • Since at around 10 hours the heart beat becomes visible under a microscope, any time after that I will be able to monitor which fish have normal rhythms and which are abnormal • The abnormal could be a sign that there is a cardiovascular anomaly caused by a particular dosage

  9. Resources • Congenital Cardiovascular Anomalies; http://www.merck.com/mmpe/sec19/ch287/ch287a.html • Baker, Fishman, Warren, Yellen; Defective “pacemaker” current (Ih) in a zebrafish mutant with a slow heart rate; http://www.pnas.org/content/94/9/4554.full?ck=nck#cited-by • Kimmel, Charles B, Stages of Embryonic Development of the Zebrafish

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