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Gram-Positive Bacilli Part Four

Gram-Positive Bacilli Part Four. MLAB 2434: Microbiology Keri Brophy -Martinez. Non-Spore Formers & Branching. Gram positive rod Non-spore formers Pleomorphic morphology Branching Genera Actinomyces Nocardia Streptomyces. Aerobic Actinomyces.

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Gram-Positive Bacilli Part Four

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  1. Gram-Positive BacilliPart Four MLAB 2434: Microbiology Keri Brophy-Martinez

  2. Non-Spore Formers & Branching • Gram positive rod • Non-spore formers • Pleomorphic morphology • Branching • Genera • Actinomyces • Nocardia • Streptomyces

  3. Aerobic Actinomyces • Member of the normal flora of mouth, head, and neck • Pathogens • Human bite wounds, eye cultures • Cervicitis and endometritis in women • Actinomycosis

  4. Aerobic Actinomyces • Gram stain • Gram positive rod • Filamentous, branching • Can be beaded • Non acid-fast

  5. Aerobic Actinomyces • Colony morphology • Spider or granular center • Branching filaments • Takes up to 7-14 to develop • Key Biochemicals • Catalase negative • Nonmotile • Non-fermenters • Produce H2S

  6. Aerobic Actinomycetes • Nocardia • Streptomyces • Actinomadura • Gordonia • Rhodococcus • Tsukamurella • Nocardiopsis • Dermatophilus

  7. Nocardia:Clinical Significance • Pulmonary form • Mostly in immunocompromised • High fatality • Starts as lung lesion • Cutaneous • Three forms • Mycetoma • Lymphocutaneous • Superficial Sulfur granules collected from draining sinus tracts in mycetoma

  8. Nocardia:Laboratory Diagnosis • Microscopy • Gram-positive branching filaments • May show beading appearance • Verify with acid fast stain • Weakly Acid-fast Gram-stained smear of sputum showing Gram-positive branched beaded bacilli.

  9. Nocardia:Laboratory Diagnosis • Gram-positive filamentous bacilli • Suspicious for actinomycetes

  10. Nocardia:Laboratory Diagnosis • Cultural characteristics • Chalky, matte, dry, crumbly appearance • May be pigmented • Beta hemolytic • Identification • Urease positive • Catalase positive • Molecular testing

  11. Streptomyces • Habitat • Soil and decaying vegetation • Disease states • Mycetoma- a chronic, localized, painless, subcutaneous infection • Sites: head and neck • Wound & Skin infections

  12. Streptomyces:Laboratory Diagnosis • Microscopic Morphology • Gram positive rod • Branching • Spider-like • Non-acid-fast

  13. Streptomyces:Laboratory Diagnosis Morphology & Characteristics • Aerobic growth in 3-30 days • Waxy, bumpy or velvety rugose forms, yellow to orange • Will grown on SBA, mycology media and LJ media • GPR with extensive branching, chains and spores • Identification • Acid-fast= negative

  14. References • http://www.atsu.edu/faculty/chamberlain/golden2000/case5.htm • http://chesschumpion.blogspot.com/2007/03/time-to-put-on-your-thinking-caps.html • http://en.wikipedia.org/wiki/File:Bacillus_subtilis_Gram.jpg • https://labs.uhstx.com/clinical_int/dols/appb.htmlhttp://www.iccb.state.il.us/pt3/mod/science/mod_bio111/mod10/p4.html • http://www.flickr.com/photos/microbeworld/sets/72157625392265538/detail/http://www.uaz.edu.mx/histo/pathology/ed/ch_9b/c9b_clue.htm • Kiser, K. M., Payne, W. C., & Taff, T. A. (2011). Clinical Laboratory Microbiology: A Practical Approach . Upper Saddle River, NJ: Pearson Education. • Mahon, C. R., Lehman, D. C., & Manuselis, G. (2011). Textbook of Diagnostic Microbiology (4th ed.). Maryland Heights, MO: Saunders.

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