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Northeast Regional SI 2011 Yale University Group 2 Cell and Developmental Biology

Northeast Regional SI 2011 Yale University Group 2 Cell and Developmental Biology. Group 2: Cell and Developmental Biology. Tara Mann Harvard University Peter Gergen Stony Brook University Casey Roehrig Harvard University Xinnian Chen (Facilitator) University of Connecticut

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Northeast Regional SI 2011 Yale University Group 2 Cell and Developmental Biology

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  1. Northeast Regional SI 2011Yale UniversityGroup 2 Cell and Developmental Biology

  2. Group 2: Cell and Developmental Biology • Tara Mann • Harvard University • Peter Gergen • Stony Brook University • Casey Roehrig • Harvard University • Xinnian Chen (Facilitator) • University of Connecticut Ryan Draft Harvard University Debbie Spikes Stony Brook University

  3. Teachable Unit: The Cell Cycle • Audience: sophomore level class for majors (40 students as presented, but adaptable for 20-500) These students already know: • Basic genetics • Mitosis • Evolution • Class would have already covered: • The cell is the fundamental unit of life • Organelle function • DNA Replication • Transcription • Cell signaling

  4. How do you go from one cell to two cells? ordered series of regulated events

  5. How do you go from one cell to two cells? ordered series of regulated events

  6. How do you go from one cell to two cells? ordered series of regulated events accurate duplication of the DNA

  7. How do you go from one cell to two cells? ordered series of regulated events accurate duplication of the DNA division of the copies into two identical daughter cells

  8. How do you go from one cell to two cells? ordered series of regulated events accurate duplication of the DNA division of the copies into two identical daughter cells

  9. How do you go from one cell to two cells? ordered series of regulated events accurate duplication of the DNA division of the copies into two identical daughter cells

  10. The cell cycle is divided into 4 phases.

  11. (handout) How might we depict the cell cycle as a function of time? Chromosome segregation 2x Cell Mass 1x 2x DNA content 1x Time

  12. On your copy of the diagram, fill in the following cell cycle events: DNA synthesis, cytokinesis, and G1 Chromosome segregation Cytokinesis 2x Cell Mass 1x DNA Synthesis 2x DNA content 1x G1 Time

  13. Which of the boxes labeled A, B, or C represents G2? Chromosome segregation 2x Cell Mass Cytokinesis 1x 2x DNA content DNA Synthesis 1x G1 A B C Time A. B. C.

  14. Which of the boxes labeled A, B, or C represents G2? Chromosome segregation Cytokinesis 2x Cell Mass 1x DNA Synthesis 2x DNA content 1x G1 G2 Time

  15. Which of the boxes labeled A, B, or C represents G2? Chromosome segregation Cytokinesis 2x Cell Mass 1x DNA Synthesis 2x DNA content 1x G1 S G2 M Time

  16. S. pombe: a model system for studying cell cycle regulation G1 S G2 M

  17. Which cell cycle step is altered in the mutant yeast cells? • Early exit from G1 • G1-S transition is blocked • Early exit from G2 • G2-M transition is blocked Wild-Type Mutant Clicker Question! Image from http://www-bcf.usc.edu/~forsburg/main4.html

  18. Describe an experiment to distinguish between these possibilities. BRAINSTORM!!! • Early exit from G1 • G1-S transition is blocked • Early exit from G2 • G2-M transition is blocked Wild-Type Mutant Image from http://www-bcf.usc.edu/~forsburg/main4.html

  19. End of Tidbit:Where would we go from here? • Lecture will transition into cell cycle regulatory mechanisms (cyclins, checkpoints) • At the end of the lecture, a homework assignment will review the material • The subsequent lecture will explore the catastrophic consequences of dysregulation in human disease

  20. Homework Assignment Replicate a diagram for the mutant in which you show the effect on the cell cycle, cell mass, and DNA content. Wild-Type Wild-Type Mutant Mutant

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