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WP6 presentation

WP6 presentation. Quality of oats : protein and oil contents Micronutriments and avenins. AVEQ meeting BUCHAREST 19-21/10/2010. Partners for this workpackage :. France : Jean Koenig INRA Clermont-Ferrand Gérard Branlard INRA Clermont-Ferrand Audrey Didier INRA Clermont-Ferrand

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WP6 presentation

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  1. WP6 presentation Quality of oats : protein and oil contents Micronutriments and avenins AVEQ meeting BUCHAREST 19-21/10/2010

  2. Partners for this workpackage : France : Jean Koenig INRA Clermont-Ferrand Gérard Branlard INRA Clermont-Ferrand Audrey Didier INRA Clermont-Ferrand External service : Vetagro sup Agnès Piquet Estonia : Külli Annamaa Jogeva Plant Breeding Institute Romania : Danela Murariu Genebank Suceava Manuela Ibanescu Genebank Suceava AVEQ meeting BUCHAREST 19-21/10/2010

  3. Preliminary work to analyses : Dehulling of seeds Done by Alf Seplitis (Partner 2) and Mathias Hermann (Partner 3) Hand dehulling has and will be done by IHAR Radzikov and PBI (Estonia) by hand which is a quite long work Thanks to them Milling has been done by laboratories carrying out NIRS analyses (problems of sticky flour..... ) AVEQ meeting BUCHAREST 19-21/10/2010

  4. Near Infrared Spectra analysis (NIRS) On samples 2008 , have been realized for 6 locations : BUL, EST, FRA, ITA, ROM, POL, and on samples 2009 are on the way. Calibration by Kjeldahl (proteins) and (Fat) and Calibration verification have been done on 2008 samples. Calibration values will be entered in the NIRS software to produce protein and fat values. AVEQ meeting BUCHAREST 19-21/10/2010

  5. Protein contentFirst results from calibration by Kjeldahl method show a wide range of variation : within locations and looking at all locations AVEQ meeting BUCHAREST 19-21/10/2010

  6. Micronutriments • Have been realized on 122 (100 selected by PCA + 2 X 11 Standards) samples from 2008 (mineral micronutriments)on samples from french harvest. But results were not produced yet by partner (external) • Samples have been chosen on spectra from NIRS analysis (PCA) choosing more diverse accessions • Will be done on samples from France 2009 in the next months AVEQ meeting BUCHAREST 19-21/10/2010

  7. Results for avenins patterns AVEQ meeting BUCHAREST 19-21/10/2010

  8. Avenin identification Method • Avenin extraction • 1D electrophoresis Acide-Page • Allele identification for the 3 loci: Ave1, Ave2 et Ave3

  9. Identification of alleles of Ave1locus • 5 alleles

  10. Identification of alleles of Ave2locus • 6 alleles

  11. Identification of alleles of Ave3locus • 10 alleles

  12. Homogeneity (purety) of the Avena sativa patterns as revealed through avenin analysis • 22.4 % of accessions were heterogenous • Genetic heterogeneity within accession • Grain mixture within accession • 80% old accessions • 20% modern accessions

  13. Identification of avenin alleles in A. sativa

  14. Alleles repartition at locus Ave1 ( N=300, 2008)

  15. Alleles repartition at locus Ave2 ( N=300, 2008)

  16. Alleles repartition at locus Ave3 ( N=300, 2008)

  17. Thanks for your attention

  18. Overview of deliverables WP6 Deliverable number Planned completion Description Status Achieved Revised completion Who D6.1 Month 36 Protein and fat data from 1st field experiment year reported Month 46 P5 D6.2 Month 40 Database with 600 Avenin patterns X P5 D6.3 Month 44 Database with 6600 NIRS spectra Month 48 P1 D6.4 Month 48 Protein and fat data from 2nd field experiment year reported Month 48 P11 D6.5 Month 48 All analysis results available in EADB Month 48 P1 Milestone number Planned completion Description Status Achieved Revised completion Who X P5 X P5 X P5 X P11, P9 X P5 Month 48 P11 Month 48 P9,P11 Month 47 P5 AVEQ meeting BUCHAREST 19-21/10/2010

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